Structure-Function relationship of identified insect olfactory projection neurones

已鉴定的昆虫嗅觉投射神经元的结构-功能关系

• 批准号: 18222510
• 负责人: Professor Dr. Peter Kloppenburg
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/18222510?language=en
• 关键词: Structure; Function; relationship; identified; insect

The projection (output) neurones of the antennal lobe are key components of the central olfactory pathway in insects. They have multiple functions, including 1) integrating olfactory information inside the antennal lobe, 2) conveying information to the protocerebrum, and 3) specifically distributing processed olfactory information to specific sites inside the protocerebrum. Using the 3-D reconstruction and modelling tool AMIRA we aim to deduct functional neurone and network properties from quantitative reconstructions of single neurones in the context of the host network. The work will be performed on large robust insects (Periplaneta americana) which are excellent preparations for physiological studies of central olfactory information processing. The study will focus on three levels of analysis: 1) The inter-microcircuit level (interface between first- and higher-order olfactory centres), 2) the microcircuit level (inside the first- and higher-order olfactory centres) and 3) the subcellular level (morphological and functional compartmentalisation inside a single neurone). To achieve these goals we will construct a digital 3-D reference atlas of the central olfactory pathway that is inlaid in a standard brain. This reference atlas will serve as a computational framework in which detailed reconstructions of single neurones will be implemented. The goal on the cellular and subcellular level is to build 3-D reconstructions of olfactory projection neurones to provide high-resolution structural data for building quantitative/functional multicompartment models that are correlated to the neighbouring input and output neurones.

触角叶投射神经元是昆虫嗅觉中枢的重要组成部分。它们具有多种功能，包括1）在触角叶内整合嗅觉信息，2）将信息传递到前脑，以及3）将处理后的嗅觉信息专门分配到前脑内的特定部位。使用3-D重建和建模工具AMIRA，我们的目标是从宿主网络中单个神经元的定量重建中扣除功能神经元和网络特性。本工作将在大型健壮昆虫（美洲大蠊）上进行，这是中枢嗅觉信息处理生理学研究的良好准备。该研究将侧重于三个层次的分析：1）微回路间水平（一级和高级嗅觉中心之间的界面），2）微回路水平（一级和高级嗅觉中心内部）和3）亚细胞水平（单个神经元内的形态和功能区室化）。为了实现这些目标，我们将构建一个数字化的3-D参考图谱的中央嗅觉通路，是镶嵌在一个标准的大脑。这个参考图谱将作为一个计算框架，在其中将实现单个神经元的详细重建。在细胞和亚细胞水平上的目标是建立嗅觉投射神经元的3-D重建，为建立与相邻输入和输出神经元相关的定量/功能多室模型提供高分辨率的结构数据。