RIG/CAA: Functional Molecular Analysis of Variation in the Development of the Arthropod Head and Nervous System

RIG/CAA：节肢动物头部和神经系统发育变异的功能分子分析

• 批准号: 0718975
• 负责人: William Browne
• 金额: $ 17.44万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718975&HistoricalAwards=false
• 关键词: RIG; CAA; Functional; Molecular; Analysis

Through this award, Dr. Browne will investigate the intriguing and important, question of how phenotypes (physical characteristics) are linked to genotypes (genetic characteristics). The most successful group of animals in earth's history has been the arthropods. Arthropods dominate, and play key ecological roles, in our seas, land, and air and thus arthropods are particularly well suited for asking how phenotype and genotype are connected. The experiments proposed by Dr. Browne are focused on understanding the unique development of the head in the arthropod, Parhyale hawaiensi. He will address the phenotype/genotype link by employing a multidisciplinary approach combining techniques from developmental and evolutionary biology, and molecular genetics. Histological techniques will be used to investigate and describe the morphology and neuroanatomy of the head and will allow comparative analyses among various arthropods and serve as an important foundation for the interpretation of molecular experiments. He will then use gene knockdown, gene overexpression, and transgenesis techniques to determine the expression and function of two genes critical to the embryonic development of the head, brain, and eye in P. hawaiensis, Ph orthodenticle1 (Photd1) and Ph rhodopsin1 (Phrh1). Altering Photd1 gene expression, combined with the detailed analysis of the regulation of Photd1 and Phrh1, will provide information concerning the role of Photd1 in organizing early head development and the potential interaction between Photd1 and Phrh1 genes in the later development of the eye. This work in a non-insect arthropod, will enhance general knowledge of the molecular basis for biological diversity and provide information regarding arthropod nervous system ontogeny. It will further develop reagents and methodologies for the use of P. hawaiensis as a model system for understanding the evolution of developmental systems. As part of his work Dr. Browne will recruit, mentor and train underrepresented minority scientists in the fields of evolution, development, and neuroscience.

通过这个奖项，布朗博士将研究如何表型（物理特征）与基因型（遗传特征）相关联的有趣而重要的问题。 地球历史上最成功的动物群体是节肢动物。 节肢动物占主导地位，并发挥关键的生态作用，在我们的海洋，陆地和空气中，因此节肢动物特别适合询问表型和基因型是如何连接的。 布朗博士提出的实验重点是了解节肢动物Parhyale hawaiensi头部的独特发育。他将通过采用多学科方法结合发育和进化生物学以及分子遗传学的技术来解决表型/基因型的联系。 组织学技术将用于调查和描述头部的形态学和神经解剖学，并将允许各种节肢动物之间的比较分析，并作为分子实验的解释的重要基础。 然后，他将使用基因敲低，基因过表达和转基因技术来确定两个基因的表达和功能，这两个基因对夏威夷假单胞菌的头部，大脑和眼睛的胚胎发育至关重要，即Ph orthodenticle 1（Photd 1）和Ph rhodopsin 1（Phrh 1）。 改变Photd 1基因的表达，结合对Photd 1和Phrh 1的调控的详细分析，将提供有关Photd 1在组织早期头部发育中的作用以及Photd 1和Phrh 1基因在眼睛后期发育中的潜在相互作用的信息。 这项工作在非昆虫节肢动物，将提高生物多样性的分子基础的一般知识，并提供有关节肢动物神经系统个体发育的信息。它将进一步开发试剂和方法，以使用夏威夷原住民作为模型系统，了解发育系统的演变。作为他工作的一部分，布朗博士将在进化、发展和神经科学领域招募、指导和培训代表性不足的少数民族科学家。