MRI: The Core Genomics Laboratory for Teaching and Research in Biotechnology

MRI：生物技术教学和研究的核心基因组实验室

• 批准号: 0722854
• 负责人: Metin Duran
• 金额: $ 21.18万
• 依托单位: Villanova University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0722854&HistoricalAwards=false
• 关键词: MRI; Core; Genomics; Laboratory; Teaching

0722854DuranEight instruments are requested that will be utilized for genomics level bioengineering research and teaching at Villanova University, namely: a genetic analyzer, a real-time PCR system, a liquid chromatograph with UV-Vis and light scattering detectors, a cell viability analyzer, an epifluorescent microscope, a plate reader, and two freezers. These instruments will form the analytical infrastructure of a new Core Genomics Laboratory. The new laboratory and the instruments requested represent a significant upgrade from the existing laboratories currently used by the co-PIs. The capabilities brought about by the new facility and the requested instruments will allow co-PIs to incorporate modern genomics level experimental protocols into their current and future research and teaching activities in environmental microbiology, bioprocessing, mechanotransduction, and microstructured optical fiber spectroscopy-based biological sensors. This proposal has significant intellectual merit in that it will allow the co-PIs to conduct advanced research in areas which they have well-established competence through prior work. The details of the current and several planned studies that necessitate the requested instruments are presented in the later sections of this proposal. These advanced studies will help to expand the knowledge base in the co-PIs' respective fields of expertise. This proposal also has significant broader impacts. First, acquisition of the requested instruments will help develop the experimental infrastructure available to the co-PIs for research and educational purposes. By improving the ability of the co-PIs to attract funded research, more graduate students will have the opportunity to take an active role in genomics research as part of their graduate education. The co-PIs are also committed to a true integration of research into the undergraduate educational experience. Through a variety of mechanisms described in the proposal, undergraduates will have the opportunity to use the requested equipment to conduct research. Underrepresented groups, including high school students from the city of Philadelphia, will benefit as a result of the continued commitment of the co-PIs and the College of Engineering toward providing opportunities for these groups in engineering education.

0722854Duran 维拉诺瓦大学需要八台用于基因组水平生物工程研究和教学的仪器，即：一台基因分析仪、一套实时PCR系统、一台带紫外-可见光和光散射检测器的液相色谱仪、一台细胞活力分析仪、一台落射荧光显微镜、一台读板机和两台冰箱。这些仪器将构成新核心基因组实验室的分析基础设施。新实验室和所要求的仪器是对联合PI目前使用的现有实验室的重大升级。新设施和所需仪器带来的功能将使共同PI能够将现代基因组学水平的实验方案纳入其当前和未来的环境微生物学、生物加工、机械传导和基于微结构光纤光谱的生物传感器的研究和教学活动中。该提案具有重要的智力价值，因为它将允许联合首席研究员在他们通过之前的工作已确立的能力领域进行高级研究。本提案的后面部分介绍了当前和几项计划进行的研究的详细信息，这些研究需要所要求的仪器。这些高级研究将有助于扩大联合首席研究员各自专业领域的知识库。该提案还具有更广泛的影响。首先，获取所需的仪器将有助于开发可供联合 PI 用于研究和教育目的的实验基础设施。通过提高联合首席研究员吸引资助研究的能力，更多的研究生将有机会在基因组学研究中发挥积极作用，作为研究生教育的一部分。联合 PI 还致力于将研究真正融入本科生教育体验中。通过提案中描述的各种机制，本科生将有机会使用所需的设备进行研究。由于共同PI和工程学院持续致力于为这些群体提供工程教育机会，包括费城市高中生在内的代表性不足的群体将受益。