Rectification of voltage-gated plant K+ channels

电压门控设备 K 通道的整流

• 批准号: 183771926
• 负责人: Professor Dr. Ingo Dreyer
• 金额: --
• 依托单位: Centro de Bioinformatica y Simulacion Molecular
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/183771926?language=en
• 关键词: Rectification; voltage; gated; plant; K+

The family of voltage-gated K+ channels in plants plays an important role in the uptake of potassium from the soil and its distribution throughout the plant. Interestingly, members of this family are structurally very similar but functionally they segregate into inward-rectifying (Kin) channels that allow plant cells to accumulate K+ and outward-rectifying (Kout) channels that mediate K+ efflux. The reason for this divergence is unknown. We generated a synthetic Kin channel (SKIN) and a synthetic Kout channel (SKOUT) that share a high rate of identity (82.5% at the protein level, 90% at the DNA level). The design of the synthetic SKIN and SKOUT clones allowed us further to generate a cDNA library with >30.000.000 different SKIN/SKOUT chimeras. This library will be used to identify chimeras that are inward- or outward-rectifying in order to further increase the degree of identity between these two channel types. The experimental work is accompanied by in silico molecular modeling and dynamics simulations. This combined approach is novel and will allow detailed structural and functional insights into the regulation of plant K+ channels. Furthermore, it will provide informative data for the attempt to design membrane proteins with defined features at the computer.

植物中的电压门控钾通道家族在植物从土壤中吸收钾并将其分配到整个植物中起着重要作用。有趣的是，这个家族的成员在结构上非常相似，但在功能上它们分为允许植物细胞积累K+的内向整流（Kin）通道和介导K+流出的外向整流（Kout）通道。这种差异的原因尚不清楚。我们产生了一个合成的Kin通道（SKIN）和一个合成的Kout通道（SKOUT），它们具有很高的同一性（蛋白质水平为82.5%，DNA水平为90%）。合成的SKIN和SKOUT克隆的设计允许我们进一步产生具有> 30，000，000个不同的SKIN/SKOUT嵌合体的cDNA文库。该文库将用于鉴定向内整流或向外整流的嵌合体，以进一步增加这两种通道类型之间的同一性程度。实验工作是伴随着在硅片分子建模和动力学模拟。这种结合的方法是新颖的，将允许详细的结构和功能的见解，植物K+通道的调节。此外，它将提供翔实的数据，试图在计算机上设计具有定义的功能的膜蛋白。