Collaborative Research IPY: Comparative Genomic and Proteomic Survey of Major Antarctic Marine Phytoplankton: A Foundation for Polar Phytoplankton Genomics

摘要到目前为止，由于缺乏序列数据，分子工具在极地浮游植物和生物地球化学研究中的应用一直受到阻碍。该研究项目将通过使用主要表达序列标签文库(EST)调查南极水域常见的三个主要分类群，为极地海洋浮游植物基因组学和蛋白质组学奠定基础。这些表达的序列标签文库将在环境相关的条件下开发，特别是在铁限制的情况下，以改进参与重要的极地生物地球化学过程的关键基因的基因模型。此外，研究人员将利用这一广泛的测序项目，开始解决与浮游植物与碳和铁的生物地球化学循环相互作用有关的重要和及时的研究问题。PIs包括一名分子生物学家和一名环境生物无机化学家/生物化学家，两人都有最近在罗斯海的实地经验。他们的合作将把基因组学工具放在一个正在经历快速变化、在全球碳循环中发挥重要作用的极地地区。P.I.S将与一名自由撰稿人和国家公共广播电台记者合作，开发一系列故事，在当地和全国广播市场发行，内容集中在极地研究和基因组工具上。此外，一名高中教师将在其中一所参与机构接受接待。将为文特尔研究所的流动推广实验室开发一个极地基因组学模块：发现基因组学！最后，拟议的EST测序和生物信息数据管理计划将与欧盟资助的功能基因组学DIATOMICS项目协同工作，该项目涉及来自5个欧洲国家和以色列的实验室。

AbstractThe application of molecular tools to polar phytoplankton and biogeochemical studies has been hindered thus far by the lack of sequence data. The research project will create a foundation for polar marine phytoplankton genomics and proteomics, by surveying three major taxa common to Antarctic waters using primarily expressed sequence tags libraries (EST). These expressed sequence tag libraries will be developed under environmentally relevant conditions, in particular under iron limitation, to improve gene models of key genes involved in important polar biogeochemical processes. In addition, the investigators will use this extensive sequencing project to begin to address important and timely research questions related to the interactions of phytoplankton with the biogeochemical cycling of carbon and iron. The PIs include a molecular biologist and an environmental bioinorganic chemist/biochemist, both with recent field experience in the Ross Sea. Their collaboration will place genomics tools in a polar region that is undergoing rapid change and plays an important role in global carbon cycling The P.I.'s will work with a freelance journalist and National Public Radio reporter to develop a series of stories for distribution in local and national radio markets, with content focused on polar research and genomic tools. In addition, a high school teacher will be hosted at one of the participating institutions. A polar genomics module will be developed for the Venter Institute's mobile outreach laboratory: DISCOVER GENOMICS! Finally, the proposed EST sequencing and bioinformatic data management program will be synergistic and collaborative with the European Union funded functional genomics DIATOMICS project that involves laboratories from 5 European countries and Israel.