Project-based LC-MS Experiments at the Interface of Chemistry and Biology

购买了一台微型API质谱联用分析仪，使得液相色谱-质谱联用（LC-MS）分析可以通过在有机化学、生物化学和环境科学实验室进行的为期数周的以团队为基础、以项目为导向的实验引入到本科课程中。目前，LC-MS在本科课程中的纳入非常有限；大学生使用这种技术的少数例子主要局限于研究环境。在实验室中使用的实验是根据研究文献改编的，并且在实验中加入了可变性，这样实验就不会变成“食谱”式的实验。在制药科学领域，有机化学实验室的学生团队利用平行合成策略开发利多卡因类似物的小型文库，并通过LC-MS确认其反应产物。然后将这些文库与肝微粒体孵育，并用LC-MS-MS分析这些化合物的代谢率。在环境科学领域，学生进行定量分析，确定污水处理厂中使用的好氧和厌氧消化方法如何影响微生物降解洗涤剂产生的烷基酚内分泌干扰物的水平。高级生物化学实验室的学生正在研究代谢组学这一新兴领域，它旨在研究细胞代谢物的整个集合（或一个定义的子集）。生物化学专业的学生使用LC-MS来识别和量化参与细胞信号事件的脂质。这些新实验通过要求学生解决具有挑战性的相关问题，同时为他们提供回答这些问题所需的尖端仪器，从而增强了实验室体验。

Chemistry (12) The purchase of a micro API MS-MS mass analyzer is allowing coupled liquid chromatography-mass spectra (LC-MS) analysis to be introduced into the undergraduate curriculum via multi-week team based, project-oriented experiments in organic chemistry, biochemistry and environmental science laboratories. The inclusion of LC-MS in the undergraduate curriculum is very limited at this time; the few examples of undergraduates using the technique have largely been restricted to research settings. The experiments for use in the laboratories are being adapted from the research literature and variability is built into them such that the experiments will not devolve into "cookbook" type ones. Working in the area of pharmaceutical science, teams of students taking organic chemistry laboratory utilize parallel synthesis strategies to develop small libraries of lidocaine analogues, confirming their reaction products by LC-MS. These libraries are then incubated with liver microsomes and the rates of metabolism of these compounds analyzed by LC-MS-MS. In the area of environmental science, students taking quantitative analysis determine how the aerobic and anaerobic digestion methods used in wastewater treatment plants affect the levels of alkylphenol endocrine disrupters that are produced by microbial degradation of detergents. Students taking senior-level biochemistry laboratory are working in the emerging area of metabolomics, which seeks to examine the entire set (or a defined subset) of cellular metabolites. The biochemistry students use LC-MS to identify and quantify lipids involved in cellular signaling events. These new experiments are enhancing the laboratory experience by asking students to address challenging, relevant questions while giving them the cutting-edge instruments needed to answer those questions.