Project-based LC-MS Experiments at the Interface of Chemistry and Biology

化学与生物学交叉领域基于项目的 LC-MS 实验

• 批准号: 0737390
• 负责人: Timothy Peelen
• 金额: $ 15万
• 依托单位: Lebanon Valley College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2011-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0737390&HistoricalAwards=false
• 关键词: Project; based; LC; MS; Experiments

Chemistry (12) The purchase of a micro API MS-MS mass analyzer is allowing coupled liquid chromatography-mass spectra (LC-MS) analysis to be introduced into the undergraduate curriculum via multi-week team based, project-oriented experiments in organic chemistry, biochemistry and environmental science laboratories. The inclusion of LC-MS in the undergraduate curriculum is very limited at this time; the few examples of undergraduates using the technique have largely been restricted to research settings. The experiments for use in the laboratories are being adapted from the research literature and variability is built into them such that the experiments will not devolve into "cookbook" type ones. Working in the area of pharmaceutical science, teams of students taking organic chemistry laboratory utilize parallel synthesis strategies to develop small libraries of lidocaine analogues, confirming their reaction products by LC-MS. These libraries are then incubated with liver microsomes and the rates of metabolism of these compounds analyzed by LC-MS-MS. In the area of environmental science, students taking quantitative analysis determine how the aerobic and anaerobic digestion methods used in wastewater treatment plants affect the levels of alkylphenol endocrine disrupters that are produced by microbial degradation of detergents. Students taking senior-level biochemistry laboratory are working in the emerging area of metabolomics, which seeks to examine the entire set (or a defined subset) of cellular metabolites. The biochemistry students use LC-MS to identify and quantify lipids involved in cellular signaling events. These new experiments are enhancing the laboratory experience by asking students to address challenging, relevant questions while giving them the cutting-edge instruments needed to answer those questions.

化学（12）购买微型API MS-MS质量分析仪，可以通过在有机化学、生物化学和环境科学实验室中进行为期多周的基于团队的项目导向实验，将耦合液相色谱-质谱（LC-MS）分析引入本科课程。 LC-MS在本科课程中的应用目前非常有限;本科生使用该技术的少数例子在很大程度上仅限于研究环境。 供实验室使用的实验是根据研究文献改编的，其中有可变性，这样实验就不会变成“食谱”式的实验。 在制药科学领域，学生团队采取有机化学实验室利用平行合成策略开发利多卡因类似物的小型库，通过LC-MS确认其反应产物。然后将这些库与肝微粒体孵育，并通过LC-MS-MS分析这些化合物的代谢率。在环境科学领域，参加定量分析的学生确定废水处理厂使用的好氧和厌氧消化方法如何影响洗涤剂微生物降解产生的烷基酚内分泌干扰物的水平。参加高级生物化学实验室的学生正在代谢组学的新兴领域工作，该领域旨在检查细胞代谢物的整个集合（或定义的子集）。生物化学专业的学生使用LC-MS来识别和量化参与细胞信号事件的脂质。这些新的实验通过要求学生解决具有挑战性的相关问题，同时为他们提供回答这些问题所需的尖端仪器来增强实验室体验。