SBIR Phase I: A versatile valveless mutlichannel parallel bioassay system

SBIR 第一阶段：多功能无阀多通道并行生物测定系统

• 批准号: 0740847
• 负责人: Xiao-Li Su
• 金额: $ 10万
• 依托单位: BioDetection Instruments, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0740847&HistoricalAwards=false
• 关键词: SBIR; Phase; versatile; valveless; mutlichannel

This Small Business Innovation Research Phase I research project will develop a versatile high throughput bioassay system. The proposed system will be built on a patent-pending biosensing technology that has been exclusively licensed from the University of Arkansas. Using this technology, the company has obtained promising preliminary results for rapid and sensitive detection of food-borne pathogens, protein biomarkers, and pesticide residues. The Phase I research will focus on increasing sample throughput, reducing cross-contamination, and simplifying operation by developing a valveless mutichannel parallel bioassay system. The proposed system will consist of an automated sample/reagent delivery module, an easy-to-change multichannel biosensor cartridge, and an interface providing on-cartridge optical detection. Escherichia coli O157:H7, one of the most common and dangerous food-borne pathogens, will be used as the model target for the feasibility study. Microbial contamination of food products by pathogenic bacteria remains a major concern of our society. Food-borne illness affects millions of Americans each year and food producers are required to test their products for pathogens in a timely manner. Although conventional culture methods hypothetically allow the detection of a single cell of specific pathogens, they are extremely time-consuming, typically requiring at least 24 hours and complicated multi-steps to confirm the analysis. They also require laboratory setup and skilled personnel. The need of rapid detection of food pathogens and other important agents has posed a challenge for high throughput bioassays.

这个小企业创新研究第一阶段的研究项目将开发一种多功能的高通量生物测定系统。拟议中的系统将建立在一项正在申请专利的生物传感技术上，该技术已获得阿肯色大学的独家许可。利用这项技术，该公司已经获得了快速、灵敏地检测食源性病原体、蛋白质生物标志物和农药残留的初步结果。第一阶段的研究将重点放在提高样品吞吐量，减少交叉污染，并通过开发无阀多通道平行生物测定系统简化操作。该系统将包括一个自动样品/试剂输送模块，一个易于更换的多通道生物传感器盒，以及一个提供盒上光学检测的接口。大肠杆菌O157:H7是最常见和最危险的食源性致病菌之一，将作为可行性研究的模型靶点。致病菌对食品的微生物污染仍然是我们社会关注的主要问题。食源性疾病每年影响数百万美国人，食品生产商被要求及时检测其产品中的病原体。虽然传统的培养方法假设允许检测特定病原体的单个细胞，但它们非常耗时，通常需要至少24小时和复杂的多个步骤来确认分析。它们还需要实验室设备和熟练的人员。快速检测食品病原体和其他重要病原体的需求对高通量生物测定提出了挑战。