CRIF:MU-Purchase of a Liquid Chromatograph Time-of-Flight Mass Spectrometer

CRIF:MU-购买液相色谱飞行时间质谱仪

• 批准号: 0741868
• 负责人: Joanne Smieja
• 金额: $ 28.68万
• 依托单位: Gonzaga University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0741868&HistoricalAwards=false
• 关键词: CRIF; MU; Purchase; Liquid; Chromatograph

With support from the Chemistry Research Instrumentation and Facilities - Multiuser Instrumentation (CRIF-MU) Program, the Department of Chemistry at Gonzaga University will purchase a liquid chromatography time-of-flight mass spectrometer. Examples of undergraduate research projects that will be performed which utilize LC-TOFMS include: 1) Characterization of synthetic substrates and natural products used in quinine biosynthesis; 2) Analysis of metabolites in lipid extracts from anaerobic respiration; 3) Structure-function studies of enzymes that involve determination of protein mass and quantification of known proteins; 4) Synthetic design and characterization of a positron emission tomography (PET) diagnostic agent; 5) Development of wound care dressings for chronic wounds; and 6) Mechanism and conformational dynamics of enzymes by hydrogen-deuterium exchange measurements.Liquid chromatography with mass spectrometric detection (LC/MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at this primarily undergraduate institution, and these studies will have a solid impact in the areas of medicinal chemistry and biochemistry.

在化学研究仪器和设施-多用户仪器（CRIF-MU）计划的支持下，贡萨加大学化学系将购买一台液相色谱飞行时间质谱仪。使用LC-TOFMS进行的本科研究项目包括：1)奎宁生物合成中合成底物和天然产物的表征；2)厌氧呼吸脂质提取物代谢产物分析；3)酶的结构-功能研究，包括蛋白质质量的测定和已知蛋白质的定量；4)正电子发射断层扫描（PET）诊断剂的合成、设计与表征；5)慢性伤口护理敷料的研制；6)通过氢-氘交换测定酶的机理和构象动力学。液相色谱质谱检测（LC/MS）是一种非常强大的技术，用于分离和分析复杂的混合物。该仪器将大大加强这所以本科生为主的机构的科学研究，这些研究将在药物化学和生物化学领域产生坚实的影响。