CRIF:MU-Purchase of a Liquid Chromatograph Time-of-Flight Mass Spectrometer

在化学研究仪器和设施-多用户仪器(CRIF-MU)计划的支持下，贡萨加大学化学系将购买一台液相色谱飞行时间质谱仪。将利用LC-TOFMS进行的本科生研究项目的例子包括：1)用于奎宁生物合成的合成底物和天然产物的表征；2)厌氧呼吸脂质提取物中代谢物的分析；3)酶的结构-功能研究，涉及蛋白质质量的测定和已知蛋白质的定量；4)正电子发射断层扫描(PET)诊断剂的合成设计和表征；5)用于慢性伤口的伤口护理敷料的开发；以及6)通过氢-氚交换测量的酶的机理和构象动力学。这一仪器将大大加强这个以本科为主的机构的科学研究，这些研究将在药物化学和生物化学领域产生坚实的影响。

With support from the Chemistry Research Instrumentation and Facilities - Multiuser Instrumentation (CRIF-MU) Program, the Department of Chemistry at Gonzaga University will purchase a liquid chromatography time-of-flight mass spectrometer. Examples of undergraduate research projects that will be performed which utilize LC-TOFMS include: 1) Characterization of synthetic substrates and natural products used in quinine biosynthesis; 2) Analysis of metabolites in lipid extracts from anaerobic respiration; 3) Structure-function studies of enzymes that involve determination of protein mass and quantification of known proteins; 4) Synthetic design and characterization of a positron emission tomography (PET) diagnostic agent; 5) Development of wound care dressings for chronic wounds; and 6) Mechanism and conformational dynamics of enzymes by hydrogen-deuterium exchange measurements.Liquid chromatography with mass spectrometric detection (LC/MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at this primarily undergraduate institution, and these studies will have a solid impact in the areas of medicinal chemistry and biochemistry.