Transcriptional Regulation of Secondary Wall Biosynthesis

次生壁生物合成的转录调控

• 批准号: 0744170
• 负责人: Zheng-Hua Ye
• 金额: $ 45万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0744170&HistoricalAwards=false
• 关键词: Transcriptional; Regulation; Secondary; Wall; Biosynthesis

The objective of the project is to investigate the transcriptional network regulating secondary wall biosynthesis in plants. Secondary walls are the major constituent of tracheary elements and fibers in wood, which is the most abundant biomass produced by plants and is widely used for pulping, paper-making, and many other commodities. To make secondary walls, genes involved in the biosynthesis of secondary wall components, including cellulose, hemicellulose and lignin, need to be coordinately turned on. Therefore, it is conceivable that there exist genetic switches responsible for turning on the secondary wall biosynthetic program. Currently, little is known about the molecular mechanisms underlying the regulation of secondary wall biosynthesis during wood formation. The investigators have recently discovered that the secondary wall-associated NAC domain transcription factor SND1 is a key switch in activating the developmental program of secondary wall biosynthesis, and that SND1 regulates the expression of several other secondary wall-associated transcription factors. One of them, MYB46, was demonstrated to be a direct target of SND1, and act upstream of the transcription factors MYB85 and KNAT7 in the regulation of secondary wall biosynthesis. Based on these findings, the investigators hypothesize that a hierarchy of transcriptional factors is involved in the regulation of secondary wall biosynthesis, and plan to unravel the SND1-mediated transcriptional network by functional characterization of the secondary wall-associated, SND1-regulated transcription factors and dissection of their interrelationships in the network. Broader impacts. The results from this project will contribute to our understanding of the molecular mechanisms underlying wood formation, and the knowledge gained will potentially provide valuable tools for genetically improving the quality and quantity of wood, the most important raw materials for traditional forest products and potentially for biofuel production. The investigators will actively integrate the proposed research with teaching activities and training of graduate and undergraduate students, especially from underrepresented groups, and will make every effort to disseminate the results of the project to enhance the understanding of plant biology research and its benefits to the society.

该项目的目的是研究调控植物次生壁生物合成的转录网络。次生壁是木材中导管分子和纤维的主要组成部分，木材是植物产生的最丰富的生物质，广泛用于制浆、造纸和许多其他商品。为了形成次生壁，需要协调地启动参与次生壁组分（包括纤维素、半纤维素和木质素）生物合成的基因，因此，可以设想存在负责启动次生壁生物合成程序的基因开关。目前，对木材形成过程中次生壁生物合成调控的分子机制知之甚少。研究人员最近发现，次生壁相关NAC结构域转录因子SND1是激活次生壁生物合成发育程序的关键开关，并且SND1调节其他几种次生壁相关转录因子的表达。其中MYB 46是SND 1的直接靶基因，在转录因子MYB 85和KNAT 7的上游调控次生壁的生物合成。基于这些发现，研究人员假设，一个层次的转录因子参与调节次生壁生物合成，并计划解开SND1介导的转录网络的功能特性的次生壁相关，SND1调节的转录因子和解剖它们在网络中的相互关系。更广泛的影响。该项目的结果将有助于我们了解木材形成的分子机制，所获得的知识将有可能提供宝贵的工具，从基因上提高木材的质量和数量，木材是传统森林产品和潜在生物燃料生产的最重要原材料。研究人员将积极将拟议的研究与教学活动和研究生和本科生的培训相结合，特别是来自代表性不足的群体，并将尽一切努力传播该项目的结果，以提高对植物生物学研究及其对社会的好处的理解。