CAREER: Propagation systems for generation of chromosomally stable human embryonic stem cells

职业：用于产生染色体稳定的人类胚胎干细胞的增殖系统

• 批准号: 0744556
• 负责人: Raj Rao
• 金额: $ 40万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2014-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0744556&HistoricalAwards=false
• 关键词: CAREER; Propagation; systems; generation; chromosomally

0744556RaoThe intellectual merit of this project is the development of an integrated ex vivo system for propagation of chromosomally stable human embryonic stem cells (hESCs), and for relating gene transcription to specific microenvironmental conditions. The ex vivo propagation system developed as part of this project will meet specific expectations that aim to (i) be efficient, producing high densities and quantities of pluripotent cells; (ii) maintain stem cells? genotypic and phenotypic integrity over several generations; and finally, (iii) use only defined, animal-product free culture components. The techniques, tools and mindset required to accomplish this research program require an overlap of interdisciplinary engineering, molecular genetics and cellular biology skills. The Principal Investigator (PI) plans to increase the awareness of the power of these interdisciplinary skills by developing educational modules for students at a variety of educational levels. By working with high school teachers, public educators at museums, and faculty at primarily undergraduate universities, the PI will develop presentation and laboratory modules that will benefit high school students, undergraduate students and the general public. The broader impacts of this project are the enormous potential for communicating research findings to a wider audience and for students and the general public to get excited about the latest advances in stem cell research.

该项目的智力价值是开发了一种用于染色体稳定的人胚胎干细胞（hESC）增殖的集成离体系统，并将基因转录与特定的微环境条件相关联。作为该项目的一部分开发的离体繁殖系统将满足特定的期望，其目的是（i）高效，产生高密度和多能细胞数量;（ii）维持干细胞？基因型和表型的完整性在几个世代;和最后，（iii）只使用定义，动物产品免费的文化成分。完成这项研究计划所需的技术，工具和心态需要跨学科工程，分子遗传学和细胞生物学技能的重叠。首席研究员（PI）计划通过为不同教育水平的学生开发教育模块，提高对这些跨学科技能的认识。通过与高中教师，博物馆的公共教育工作者和主要是本科大学的教师合作，PI将开发演示和实验室模块，使高中生，本科生和公众受益。这个项目的更广泛的影响是将研究成果传达给更广泛的受众的巨大潜力，以及学生和公众对干细胞研究的最新进展感到兴奋。