The Arabidopsis SNI1 Complex Regulates Both Gene Transcription and DNA Recombination During the Defense Response

拟南芥 SNI1 复合物在防御反应期间调节基因转录和 DNA 重组

• 批准号: 0744602
• 负责人: Xinnian Dong
• 金额: $ 68万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0744602&HistoricalAwards=false
• 关键词: Arabidopsis; SNI1; Complex; Regulates; Both

Systemic acquired resistance is an immune response in plants that confers long-lasting resistance against a broad spectrum of pathogens. It is controlled by NPR1 and SNI1, which serve as the "on" and "off" switches for expression of proteins with antimicrobial activities, respectively. Pathogen infection also triggers an increase in DNA recombination to generate new traits for better adaptation to the selective pressure. The investigators of this project identified several additional proteins affecting both antimicrobial gene expression and DNA recombination and showed that they physically interact with SNI1. Through this project, these investigators will apply molecular genetic and genomic approaches to study the SNI1 protein complex and elucidate how this complex regulates both gene transcription and DNA recombination during plant immune responses. This project pushes the boundaries between studies of plant defense-related gene expression and chromosome structure and function in all higher organisms. For example, one of the proteins of interest is BRCA2, which is associated with familial breast cancer in humans. But the molecular function of BRCA2 is not well known. Studies of BRCA2 function in plant defense may shed new light on how this protein functions in general. The success of this project will also have a significant impact on agriculture in controlling crop disease and benefit the efforts to project the environment. The broader impacts of this project also include excellent training opportunities for postdocs, graduate and undergraduate students in molecular genetics and genomics. It will allow the principle investigator of the project to continue serving as a mentor in a program for minority undergraduate summer research and a panelist in the University Women in Science and Engineering program.

系统获得性抗性是植物体内的一种免疫反应，对广泛的病原体具有持久的抗性。它由NPR1和SNI1控制，它们分别作为具有抗菌活性的蛋白质表达的“开”和“关”开关。病原体感染也会触发DNA重组的增加，以产生新的特征，以更好地适应选择压力。该项目的研究人员发现了另外几种影响抗菌基因表达和DNA重组的蛋白质，并表明它们与SNI1存在物理上的相互作用。通过这个项目，这些研究人员将应用分子遗传学和基因组学方法来研究SNI1蛋白复合体，并阐明该复合体如何在植物免疫反应中调节基因转录和DNA重组。该项目推动了所有高等生物中与植物防御相关的基因表达与染色体结构和功能的研究之间的界限。例如，其中一种感兴趣的蛋白质是BRCA2，它与人类家族性乳腺癌有关。但是BRCA2的分子功能还不是很清楚。对BRCA2在植物防御中的功能的研究可能会为该蛋白的总体功能提供新的线索。该项目的成功还将对农业在控制作物病害方面产生重大影响，并有利于环境项目的努力。该项目的更广泛影响还包括为分子遗传学和基因组学的博士后、研究生和本科生提供极好的培训机会。它将允许该项目的首席研究员继续担任少数族裔本科生暑期研究项目的导师和大学女性科学与工程项目的小组成员。