Arabidopsis 2010: Global Analysis of Plant Disease Resistance Pathways

拟南芥 2010：植物抗病途径的全球分析

• 批准号: 0929226
• 负责人: Xinnian Dong
• 金额: $ 404.28万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0929226&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Global; Analysis; Plant

Unlike animals, plants lack a specialized immune system. Plant cells while performing their normal functions also have to defend against pathogen infection. This makes it difficult to study plant immunity independently of other biological processes. To fully understand how plants respond to pathogen challenge, a 'systems biology' approach is most likely to uncover key regulatory networks underlying plant immunity and how they interface with processes like growth and development. The investigators of this project are in a unique position to carry out systems biology studies of plant immunity because of their pioneering work in using the model organism Arabidopsis in identifying essential components for all the major plant defense response pathways and in establishing a framework for placing these diverse components in an integrated network. However, there are still many parts missing in this network, including the identities of resistance output components and their regulators. Another major challenge is to understand the interconnections between the different immune responses. This project aims to identify these missing components and to build not only a static network but also dynamic models of the plant immune system. The results obtained from this project will be published in peer-reviewed journals and disseminated at meetings. Mutants and transgenic lines will be deposited in the Arabidopsis Biological Resource Center. Large data sets will be deposited in public databases (e.g., NCBI's GEO for microarray and RNA seq data) as well as in the project database (http://ausubellab.mgh.harvard.edu/nsf2010/index.jsp) prior to or at the time of publication. Understanding plant adaptive mechanisms to environmental stress is one of the objectives of the NSF2010 Project. The success of this project will lead to better, environmentally friendly strategies for controlling crop diseases and will provide excellent training opportunities for undergraduates, graduate students, and postdoctoral fellows in classical genetics, phytopathology as well as cutting-edge genomics and bioinformatics technologies.

与动物不同，植物缺乏专门的免疫系统。植物细胞在行使其正常功能的同时，也必须防御病原体的感染。这使得很难独立于其他生物过程来研究植物免疫。为了充分了解植物如何应对病原体的挑战，“系统生物学”方法最有可能揭示植物免疫的关键调控网络，以及它们如何与生长和发育等过程相互作用。该项目的研究人员在开展植物免疫系统生物学研究方面处于独特的地位，因为他们开创性地利用模式生物拟南芥来识别所有主要植物防御反应途径的基本成分，并建立了将这些不同成分置于综合网络中的框架。然而，在该网络中仍有许多缺失的部分，包括电阻输出元件及其调节器的身份。另一个主要挑战是了解不同免疫反应之间的相互联系。本项目旨在确定这些缺失的成分，并建立植物免疫系统的静态网络和动态模型。从这个项目获得的结果将发表在同行评议的期刊上，并在会议上传播。突变体和转基因系将存放在拟南芥生物资源中心。大型数据集将在出版之前或出版时存入公共数据库（例如NCBI的GEO微阵列和RNA序列数据）以及项目数据库（http://ausubellab.mgh.harvard.edu/nsf2010/index.jsp）。了解植物对环境胁迫的适应机制是NSF2010项目的目标之一。该项目的成功将导致更好的、环境友好的作物病害控制策略，并将为本科生、研究生和博士后提供经典遗传学、植物病理学以及尖端基因组学和生物信息学技术方面的良好培训机会。