Collaborative Research: Oxidative Stress, Telomere Dynamics and Aging in a Free-living Organism

合作研究：自由生物体的氧化应激、端粒动力学和衰老

• 批准号: 0744753
• 负责人: David Winkler
• 金额: $ 14.73万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0744753&HistoricalAwards=false
• 关键词: Collaborative; Research; Oxidative; Stress; Telomere

This research is designed to identify the mechanisms that comprise the process of aging and to demonstrate its fitness consequences within a natural population of free-living vertebrates. Birds are noteworthy for having longer life spans than similar sized mammals. Tree swallows are the model system used because a large number of known-aged individuals can be repeatedly captured and sampled from year to year, and their reproductive success is easily measured across their life span. This research examines natural variation in physiological factors associated with aging in free-living birds. Longitudinal samples from individual tree swallows will be used to track oxidative stress, dysregulation of telomeres (chromosomal caps that protect coding DNA but shorten with cell division), and immune function decline. The extent to which individual variation in physiological mechanisms accounts for observed differences in survival and reproductive success will allow the researchers to identify the relative importance of these alternative pathways through which aging may occur. Samples from a cross-section of birds of different ages will identify how population averages for each physiological trait change with age, and comparison of the longitudinal and cross sectional samples will separate effects of individual aging from effects of selection between individuals with different trait values. The research will include an experiment designed to increase metabolic rates of individual swallows to test the hypothesis that oxidative metabolism and the attendant production of free radicals is causally important to the progression of aging and telomere shortening. It will provide laboratory and field training of high school, undergraduate, and graduate students as well as involvement by high school science teachers. This research will enable researchers interested in aging to link phenotypic trait values and risks of mortality, and identify evolutionarily important mechanisms of aging. In addition, this research will provide new tools to substantially strengthen the study of trade-offs in life history strategies.

这项研究旨在确定组成衰老过程的机制，并在自由生活的脊椎动物的自然种群中证明其健身后果。值得注意的是，鸟类的寿命比类似大小的哺乳动物长。树燕是使用的模型系统，因为大量已知年龄的个体可以每年重复捕获和采样，并且它们的繁殖成功很容易在其整个生命周期中测量。这项研究探讨了与自由生活的鸟类衰老相关的生理因素的自然变化。来自个体树燕的纵向样本将用于跟踪氧化应激、端粒（保护编码DNA但随着细胞分裂而缩短的染色体帽）失调和免疫功能下降。生理机制的个体差异在多大程度上解释了观察到的生存和生殖成功的差异，这将使研究人员能够确定这些替代途径的相对重要性，通过这些途径可能发生衰老。来自不同年龄鸟类的横截面样本将确定每个生理性状的群体平均值如何随年龄变化，纵向和横截面样本的比较将分离个体老化的影响与具有不同性状值的个体之间的选择的影响。这项研究将包括一项旨在增加个体燕子代谢率的实验，以检验氧化代谢和随之产生的自由基对衰老和端粒缩短的进展具有重要因果关系的假设。它将为高中、本科和研究生提供实验室和实地培训，并由高中科学教师参与。这项研究将使对衰老感兴趣的研究人员能够将表型特征值与死亡风险联系起来，并确定衰老的进化重要机制。此外，这项研究将提供新的工具，大大加强生活史策略的权衡研究。