CAREER: Genomic Analysis of Pheromone-Mediated Behavior

职业：信息素介导行为的基因组分析

• 批准号: 0746338
• 负责人: Christina Grozinger
• 金额: --
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0746338&HistoricalAwards=false
• 关键词: CAREER; Genomic; Analysis; Pheromone; Mediated

Chemical communication is a complex process in which a chemical signal is translated into a distinct behavioral or physiological response. The environmental context, physiological state, or genotype of the receiving animal can profoundly affect how the animal responds, but the molecular bases for this modulation are largely unknown. This project is designed to study the genetic and physiological factors that modulate how honey bees respond to queen mandibular pheromone (QMP), the main pheromonal regulator of bee behavior and colony organization. QMP is unusual in that it induces a variety of responses. These studies will characterize the effects of physiological factors and genetic variation on three responses, but also determine if primer and releaser pheromone responses are coupled, and if specific brain gene expression patterns are associated with different responses. This project will provide insight into how an external chemical cue is translated into a behavioral or physiological response by the brain, and will also address the physiological, genotypic and evolutionary factors that modulate these processes. The studies will serve as a platform for integrated education, training and outreach activities. In particular the behavioral assays will be the cornerstone of a program aimed at engaging high school students in hands-on scientific research, by allowing them to create, design, and analyze their own experiments using honey bees. The microarray studies will be used in two new courses, Insect Neurogenomics and Molecular Techniques in Ecology and Evolution, to both demonstrate to students the opportunities available in interdisciplinary molecular research on non-genetic model systems and to familiarize them with cutting edge molecular techniques. Lastly, these studies will help build and support an active research program that engages K-12 students, undergraduates, graduate students and post-doctoral researches, and integrates their work with a number of collaborative ventures both within and between departments at NCSU, and nationally and internationally.

化学通信是一个复杂的过程，其中化学信号被转化为独特的行为或生理反应。 接收动物的环境环境，生理状态或基因型可以深刻影响动物的反应，但是这种调节的分子碱基在很大程度上尚不清楚。 该项目旨在研究调节蜂蜜蜜蜂对蜜牛女王的信息素（QMP）的遗传和生理因素，后者是蜜蜂行为和菌落组织的主要信息符号调节剂。 QMP是不寻常的，因为它引起了各种反应。 这些研究将表征生理因素和遗传变异对三个反应的影响，但也确定底漆和释放剂的信息素反应是否耦合，以及特定的脑基因表达模式是否与不同的反应相关。 该项目将提供有关如何将外部化学提示转化为大脑行为或生理反应的洞察力，还将解决调节这些过程的生理，基因型和进化因素。 这些研究将成为综合教育，培训和外展活动的平台。 特别是，行为分析将是一项计划的基石，旨在通过允许他们使用蜜蜂创建，设计和分析自己的实验来吸引高中生进行动手科学研究。 微阵列研究将用于两种新课程：生态学和进化中的昆虫神经基因组学和分子技术，都向学生展示了非遗传模型系统跨学科分子研究的机会，并使它们熟悉最先进的分子技术。 最后，这些研究将有助于建立和支持一项积极的研究计划，该计划与K-12学生，本科生，研究生和博士后研究相关，并将其与NCSU内部和部门之间以及国内和国际部门之间的许多合作企业相结合。