COLLABORATIVE RESEARCH: Molecular Mechanisms Underpinning The Kin Selection Theory Of Intragenomic Conflict

合作研究：基因组内冲突的亲缘选择理论的分子机制

• 批准号: 1616257
• 负责人: Christina Grozinger
• 金额: $ 38.95万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1616257&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; Molecular; Mechanisms; Underpinning

This project seeks to understand the genetic mechanisms regulating selfish and cooperative behaviors in animals, using honey bees as a model system. Previous studies showed that, in honey bees, genes inherited from the father promote selfish behavior, while the genes inherited from the mother promote cooperative behavior. The current project will use advanced genomic tools to unravel the molecular mechanisms that mediate this conflict between the father's and mother's genes, and provide a framework for understanding how the interactions between these genes generate behavior. The insights gained and methodology developed for these studies can be easily applied to explore these questions in other social animals. This project will be used as charismatic platform to increase understanding of social behavior in animals, how genes produce behavior, and why genes may be in conflict with each other, in K-12 students and teachers, the public, undergraduate and graduate students. This project evaluates the function and regulation of intragenomic conflict in mediating social behavior, using honey bees as a model system. The kin selection theory of intragenomic conflict predicts that, in honey bees, patrigenes (inherited from the father) favor selfish behavior while matrigenes (inherited from the mother) favor altruistic behavior. Previous studies provided the first empirical support for this prediction. The current research will expand on these studies by: (1) confirming that patrigene expression mediates selfish behavior by developing new methodology for allele-specific manipulation of transcript abundance; (2) determining if parent-specific gene expression (PSGE) patterns are labile and vary according to tissue and context, while still corresponding to the predictions of kin selection theory; and (3) evaluating the extent of parent-specific DNA methylation (PSDM) across the genome and its role in regulating PSGE. These studies will integrate genetic crosses, behavior, physiology, RNAi approaches, whole transcriptome sequencing, bisulfite sequencing, bioinformatics, and statistical genomics, and open the door to examining intragenomic conflict as a pervasive force that can shape a myriad of traits related to fitness in social groups.This project is co-funded by the Genetic Mechanisms Program in the Division of Molecular and Cellular Biosciences and by the Animal Behavior Program in the Division of Integrative Organismal Systems.

该项目旨在了解使用蜜蜂作为模型系统的动物自私和合作行为的遗传机制。 先前的研究表明，在蜜蜂中，从父亲那里继承的基因促进了自私的行为，而从母亲继承的基因促进了合作行为。 当前的项目将使用先进的基因组工具来揭示介导父母基因之间这种冲突的分子机制，并提供一个框架，以了解这些基因之间的相互作用如何产生行为。 获得的见解和为这些研究开发的方法可以轻松地用于在其他社交动物中探索这些问题。 该项目将被用作具有魅力的平台，以增加对动物社会行为的理解，基因如何产生行为以及为什么基因在K-12学生和老师，公众，本科生和研究生中可能相互冲突。该项目评估了使用蜜蜂作为模型系统，评估基因组内冲突的功能和调节。基因组内冲突的亲属选择理论预测，在蜜蜂中，patrigenes（源自父的继承）赞成自私的行为，而矩阵（从母亲继承）则赞成利他行为。先前的研究为该预测提供了第一个经验支持。 当前的研究将通过：（1）证实，帕特里格表达通过为特定于等位基因的特定于转录本丰度操纵的方法来介导自私的行为； （2）确定母体特异性基因表达（PSGE）模式是否不稳定并根据组织和上下文而变化，同时仍然与亲属选择理论的预测相对应； （3）评估整个基因组中母体特异性DNA甲基化（PSDM）的程度及其在调节PSGE中的作用。 这些研究将整合遗传杂交，行为，生理学，RNAI方法，整个转录组测序，亚硫酸盐测序，生物信息学和统计基因组学，并为基因组内的冲突开辟了一扇门，以研究与社交组相关的摩尔属性和跨性别的概念的概念。生物科学和综合有机系统划分的动物行为计划。