Development of Novel Models for the Growth of Tissue Engineered Cartilage

0602608 barabino1350万成年人因骨关节炎或损伤而遭受软骨损伤。组织工程为软骨组织结构的发展提供了一种有前途的方法，用于替代或修复因损伤或疾病而受损的天然软骨。该方法利用生物反应器提供模拟体内调节组织生长发育的生化和机械信号的体外环境。该项目的目标是优化和表征一种新型的、为软骨组织工程设计的“波浪壁”生物反应器的条件。长期目标是优化用于开发工程软骨结构的生物处理条件，以便为软骨修复产生改进的替代品。水动力环境，培养过程中的氧气可用性，生物反应器内支架的空间位置，以及生长因子的使用，将被检查并与支架组织的生长和发育相关。教育和推广部分包括PI在代表性不足的群体中的出色领导作用，包括从K-12到教师级别的各个级别。

0602608Barabino13.5 million adults suffer from cartilage damage due to osteoarthritis or injury. Tissue engineering presents a promising method for the development of cartilage tissue constructs for replacement or repair of native cartilage damaged by injury or disease. Bioreactors are utilized in this method to provide in vitro environments that mimic the in vivo biochemical and mechanical signals that regulate tissue growth and development. The goal of this project is to optimize and characterize conditions in a novel, "wavy walled" bioreactor designed for cartilage tissue engineering. The long-term goal is to optimize the bioprocessing conditions used in developing engineered cartilage constructs in order to generate improved alternatives for cartilage repair. The hydrodynamic environment, oxygen availability during cultivation, scaffold spatial position within the bioreactor, and the use of growth factors, will be examined and correlated to scaffold tissue growth and development. The educational and outreach components include excellent leadership role of the PI among underrepresented groups and includes various levels, K-12 through faculty level.