EAGER: Novel prognostic microfluidic systems for cell separation from whole blood

BarabinoCBET -1229954在拟议的研究中，微流体技术独特地与血液分离原理结合应用，以满足对简化预后设备的迫切需求，以帮助评估疾病进展和监测治疗。 这种方法的创新在于在单个微流体系统（芯片）内利用双重分离技术，并使用单个全血样品来分离血细胞和循环内皮细胞。 在血液疾病中，如镰状细胞病，这些细胞持有与疾病病理学相关的重要信息。 在单个芯片上从外周血中分离多种细胞类型代表了微流体技术的进步，该技术在可靠评估疾病预后方面具有巨大潜力。疾病状态的诊断和监测对健康至关重要。 微流体等工程工具和系统为开发简单系统提供了前所未有的机会，这些系统可供个人用于评估疾病预后和监测治疗。 拟议的研究将联合收割机现有的微流体和分离技术结合起来，从血液中分离细胞成分，并提供一种具有预测功能的设备，用于非常复杂的血液疾病。 这些与疾病病理学相关的细胞成分的分离和分析可能会导致经病理学验证的生物标志物，并增强评估和治疗策略。 少数民族在翻译研究中的代表性不足，拟议的项目通过与PI领导的生物医学创新和翻译学者（BITS）计划合作解决了这一差距。BITS旨在为代表性不足的少数民族研究生和博士后提供创新，转化研究和创业方面的教育和指导。

BarabinoCBET - 1229954In the proposed studies microfluidic technologies are uniquely applied in combination with blood separation principles to meet a pressing need for simplified prognostic devices to aid in assessing disease progression and monitoring treatment. The innovation in this approach is the utilization of dual separation techniques within a single microfluidic system (chip) and using a single whole blood sample to isolate blood cells and circulating endothelial cells. In blood disorders, such as sickle cell disease, these cells hold important information related to disease pathology. The separation of multiple cell types from peripheral blood on a single chip represents an advancement in microfluidic technology that has significant potential for reliable assessment of disease prognosis.Diagnosis and monitoring of disease states is critically important to health. Engineering tools and systems, such as microfluidics, provide unparalleled opportunities for the development of simple systems that can be used by individuals to assess disease prognosis and monitor treatment. The proposed studies combine existing microfluidic and separation technologies to separate cellular components from blood and deliver a device with predictive capabilities for a very complex blood disorder. Separation and analysis of these cellular components, which are linked to disease pathology, are likely to lead to prognostically validated biomarkers and enhance strategies for assessment and treatment. Minorities are underrepresented in translational research and the proposed project addresses this gap through partnership with the Biomedical Innovation and Translation Scholars (BITS) program led by the PI. BITS is designed to provide education and mentoring to underrepresented minority graduate students and postdocs on innovation, translational research and entrepreneurship.