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Investigation about the appearance of epitopes in milk protein variants, their allergenicity and potential use in human nutrition.

研究乳蛋白变体中表位的出现、其过敏性以及在人类营养中的潜在用途。

基本信息

批准号：
185846625
负责人：
Professor Dr. Georg Erhardt
金额：
--
依托单位：
Lehrstuhl für Tierzucht und Haustiergenetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/185846625?language=en
关键词：
Investigation about appearance epitopes milk

项目摘要

Within the caseins and whey proteins, a wide range of genetic variants have been described for cow but also for other species (sheep, goat, water buffalo, camel). Milk protein variants are an important aid in animal breeding and are already included in breeding strategies. However, their importance regarding human nutrition and in particular the allergenic potential has not yet been adequately exploited. The results of the first phase of the project showed that the genetic polymorphism influence the arising peptide pattern of several casein variants and thus modifications in their allergenic epitopes occurred. Furthermore, it could be demonstrated by means of microarray immunoassay and sera from cows milk allergic patients that milk protein variants differ in their allergenic potential. With the renewal proposal, the analyses of gastrointestinal digestion and allergenicity should be extended to the beta-lactoglobulin variants A, B and C, which have been identified within the screening of the different breeds during the first phase of the project. There is an increasing interest in the use of milk from other species as an alternative protein source for patients with cows milk allergy. Thus, in addition to the epitopes of milk proteins from cow, the corresponding epitopes of casein and whey protein variants from sheep, goat, water buffalo and camel should be included in the microarray immunoassays to determine differences in the allergenicity between these species and to examine the influence of the genetic polymorphism on the IgE-binding properties of these epitopes.

在酪蛋白和乳清蛋白中，已经描述了奶牛以及其他物种（绵羊，山羊，水牛，骆驼）的广泛遗传变异。乳蛋白变异是动物育种的重要辅助手段，已被纳入育种策略。然而，它们对人类营养的重要性，特别是对引起过敏的可能性的重要性尚未得到充分利用。该项目的第一阶段结果表明，遗传多态性影响了几种酪蛋白变体产生的肽模式，从而发生了其致敏表位的修饰。此外，通过微阵列免疫分析和牛奶过敏患者的血清，可以证明牛奶蛋白变体在致敏潜力方面存在差异。根据更新建议，应将胃肠道消化和过敏原分析扩展到β -乳球蛋白变体A， B和C，这些变体在项目第一阶段的不同品种筛选中已被确定。有越来越多的人对使用其他物种的牛奶作为牛奶过敏患者的替代蛋白质来源感兴趣。因此，除了奶牛的乳蛋白表位外，还应将绵羊、山羊、水牛和骆驼的酪蛋白和乳清蛋白变异的相应表位纳入微阵列免疫分析，以确定这些物种之间的致敏性差异，并研究遗传多态性对这些表位的ige结合特性的影响。