Intramolecular indicator-displacement assays (IIDA) for multianalyte sensing

获得有机和大分子化学项目的奖项后，Pavel Anzenbacher教授利用由阴离子染料连接到受体片段组成的单分子组装来制备用于分析多种阴离子分析的传感器阵列。在静息状态下，阴离子染料被受体结合，在分析物存在下，染料从受体移位。受体结合染料和自由染料具有不同的光学性质（颜色、荧光），指示位移传感器将改变颜色或荧光，从而表明分析物的存在。染料位移传感器将被浇铸成多传感器阵列，或者作为惰性聚合物的混合物，或者共价附着在珠子上，形成类似生物环境（蛋白质、膜）的极性亲水性环境。传感器的性能（检测极限、动态范围、线性度、鉴别能力等）将在水中和竞争电解质中进行测试，并应用于生物流体的分析。结果将与离子色谱法作为参考方法得到的数据进行比较。传感数据将使用模式识别方法进行处理，定量分析将使用人工神经网络分析进行。更广泛的影响：这种用于复杂阴离子分析的传感器设计可能会导致多种阴离子以及其他复杂环境（如生物流体）分析的发展。发展这一概念，并回答有关受体和聚合物载体在传感过程中的协同作用的相关基本问题，对科学界来说是至关重要的。离子在有机准单层和薄膜中的输运信息将有助于对人工离子输运通道的认识。技术影响可用于教育、化学工业（水环境分析）和医学（例如生物流体中的药物分析）等多个领域。该方法的易于制备和灵活性被认为是一个主要优势，因为它可以快速筛选和低成本的材料和传感器设备。

With this award from the Organic and Macromolecular Chemistry Program Prof. Pavel Anzenbacher seeks the preparation of sensor arrays for analyzing multiple anionic analytes utilizing single-molecule assembly composed of an anionic dye tethered to a receptor moiety. While in the resting state the anionic dye is bound by the receptor, in the presence of an analyte the dye is displaced from the receptor. The receptor-bound and free dye have different optical properties (color, fluorescence), the Indicator-displacement sensor will change color or fluorescence, thus signaling the presence of analyte.The dye-displacement sensors will be cast into multi-sensor arrays either as blends with the inert polymer or covalently attached to beads designed to form polar hydrophilic environment that resembles biological environments (proteins, membranes). The performance (limit of detection, dynamic range, linearity, discriminatory capacity, etc.) of the sensors will be tested in water and in competing electrolytes, and applied to analysis in biological fluids. The results will be compared to data obtained by ion chromatography as a reference method. The sensing data will be processed using pattern recognition methods, quantitative analyses will be performed using artificial neural network analyses.Broader Impacts: This design of sensors for complex anionic analytes could result in the development of assays for multiple anions as well as other analytes in complex environments such as biological fluids. Developing this concept and answering related fundamental questions regarding the synergy between receptors and polymeric carriers in the sensing process is of fundamental importance as well as of interest to the scientific community. The information on ion transport in organic quasi-monolayers and thin films will contribute to the knowledge on artificial ion-transport channels. Technological implications could be harnessed in diverse fields including education, chemical industry (aqueous environments analysis), and medicine (e.g. drug analysis in biological fluids). The ease of preparation and flexibility of the method is perceived as a major asset as it would allow for rapid screening and low-cost materials and sensor devices.