Intramolecular indicator-displacement assays (IIDA) for multianalyte sensing

Pavel Anzenbacher教授获得了有机和高分子化学项目的这一奖项，他致力于利用由连接到受体部分的阴离子染料组成的单分子组件来制备传感器阵列，用于分析多个阴离子分析物。在静止状态下，阴离子染料与受体结合，在分析物存在的情况下，染料从受体上移位。受体结合的染料和游离的染料具有不同的光学性质(颜色、荧光)，指示剂位移传感器会改变颜色或荧光，从而发出分析物存在的信号。染料位移传感器将被浇铸成多传感器阵列，要么作为惰性聚合物的混合物，要么共价连接到旨在形成类似生物环境(蛋白质、膜)的极性亲水环境的珠子上。性能(检测极限、动态范围、线性度、判别能力等)传感器的一部分将在水和竞争电解质中进行测试，并应用于生物液体中的分析。结果将与离子色谱法获得的数据作为参考方法进行比较。传感数据将使用模式识别方法进行处理，定量分析将使用人工神经网络分析。广泛的影响：这种针对复杂阴离子分析物的传感器设计可能导致开发多个阴离子以及复杂环境中的其他分析物的分析方法，如生物流体。发展这一概念并回答有关受体和聚合物载体在传感过程中的协同作用的相关基本问题具有基本的重要性，也是科学界感兴趣的。有关离子在有机准单分子膜和薄膜中传输的信息将有助于人们对人工离子传输通道的认识。可在教育、化学工业(水环境分析)和医学(例如，生物液体中的药物分析)等不同领域利用技术影响。该方法的准备容易和灵活性被认为是一项主要资产，因为它将允许快速筛选和低成本的材料和传感器设备。

With this award from the Organic and Macromolecular Chemistry Program Prof. Pavel Anzenbacher seeks the preparation of sensor arrays for analyzing multiple anionic analytes utilizing single-molecule assembly composed of an anionic dye tethered to a receptor moiety. While in the resting state the anionic dye is bound by the receptor, in the presence of an analyte the dye is displaced from the receptor. The receptor-bound and free dye have different optical properties (color, fluorescence), the Indicator-displacement sensor will change color or fluorescence, thus signaling the presence of analyte.The dye-displacement sensors will be cast into multi-sensor arrays either as blends with the inert polymer or covalently attached to beads designed to form polar hydrophilic environment that resembles biological environments (proteins, membranes). The performance (limit of detection, dynamic range, linearity, discriminatory capacity, etc.) of the sensors will be tested in water and in competing electrolytes, and applied to analysis in biological fluids. The results will be compared to data obtained by ion chromatography as a reference method. The sensing data will be processed using pattern recognition methods, quantitative analyses will be performed using artificial neural network analyses.Broader Impacts: This design of sensors for complex anionic analytes could result in the development of assays for multiple anions as well as other analytes in complex environments such as biological fluids. Developing this concept and answering related fundamental questions regarding the synergy between receptors and polymeric carriers in the sensing process is of fundamental importance as well as of interest to the scientific community. The information on ion transport in organic quasi-monolayers and thin films will contribute to the knowledge on artificial ion-transport channels. Technological implications could be harnessed in diverse fields including education, chemical industry (aqueous environments analysis), and medicine (e.g. drug analysis in biological fluids). The ease of preparation and flexibility of the method is perceived as a major asset as it would allow for rapid screening and low-cost materials and sensor devices.