Intramolecular indicator-displacement assays (IIDA) for multianalyte sensing

用于多分析物传感的分子内指示剂位移测定 (IIDA)

• 批准号: 0750303
• 负责人: Pavel Anzenbacher
• 金额: $ 39万
• 依托单位: Bowling Green State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2013-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0750303&HistoricalAwards=false
• 关键词: Intramolecular; indicator; displacement; assays; IIDA

With this award from the Organic and Macromolecular Chemistry Program Prof. Pavel Anzenbacher seeks the preparation of sensor arrays for analyzing multiple anionic analytes utilizing single-molecule assembly composed of an anionic dye tethered to a receptor moiety. While in the resting state the anionic dye is bound by the receptor, in the presence of an analyte the dye is displaced from the receptor. The receptor-bound and free dye have different optical properties (color, fluorescence), the Indicator-displacement sensor will change color or fluorescence, thus signaling the presence of analyte.The dye-displacement sensors will be cast into multi-sensor arrays either as blends with the inert polymer or covalently attached to beads designed to form polar hydrophilic environment that resembles biological environments (proteins, membranes). The performance (limit of detection, dynamic range, linearity, discriminatory capacity, etc.) of the sensors will be tested in water and in competing electrolytes, and applied to analysis in biological fluids. The results will be compared to data obtained by ion chromatography as a reference method. The sensing data will be processed using pattern recognition methods, quantitative analyses will be performed using artificial neural network analyses.Broader Impacts: This design of sensors for complex anionic analytes could result in the development of assays for multiple anions as well as other analytes in complex environments such as biological fluids. Developing this concept and answering related fundamental questions regarding the synergy between receptors and polymeric carriers in the sensing process is of fundamental importance as well as of interest to the scientific community. The information on ion transport in organic quasi-monolayers and thin films will contribute to the knowledge on artificial ion-transport channels. Technological implications could be harnessed in diverse fields including education, chemical industry (aqueous environments analysis), and medicine (e.g. drug analysis in biological fluids). The ease of preparation and flexibility of the method is perceived as a major asset as it would allow for rapid screening and low-cost materials and sensor devices.

凭借有机和大分子化学计划的这一奖项，Pavel Anzenbacher教授寻求利用由连接到受体部分的阴离子染料组成的单分子组装来制备用于分析多种阴离子分析物的传感器阵列。当处于静止状态时，阴离子染料被受体结合，在分析物存在下，染料从受体置换。受体结合的和游离的染料具有不同的光学性质（颜色、荧光），指示剂位移传感器将改变颜色或荧光，从而发出分析物存在的信号。染料位移传感器将被铸造成多传感器阵列，作为与惰性聚合物的混合物或共价连接到设计成形成类似于生物环境（蛋白质、膜）的极性亲水环境的珠粒。性能（检测限、动态范围、线性、鉴别能力等）的传感器将在水中和竞争电解质中进行测试，并应用于生物液体的分析。将结果与通过离子色谱法（作为参比方法）获得的数据进行比较。传感数据将使用模式识别方法进行处理，定量分析将使用人工神经网络analysis.Broader影响：这种复杂阴离子分析物的传感器设计可能会导致在复杂环境中，如生物流体中的多种阴离子以及其他分析物的检测方法的发展。发展这一概念，并回答相关的基本问题，在传感过程中的受体和聚合物载体之间的协同作用是至关重要的，以及感兴趣的科学界。有机准单分子膜和薄膜中离子输运的信息将有助于对人工离子输运通道的认识。技术影响可用于不同领域，包括教育、化学工业（水环境分析）和医学（例如生物液体中的药物分析）。该方法的易于制备和灵活性被认为是一个主要优点，因为它将允许快速筛选和低成本的材料和传感器设备。