IDBR: Development of a Multivariate Hyper-Spectral Instrument for High-Resolution Chemical Imaging of Cell Structure and Dynamics

IDBR：开发用于细胞结构和动力学高分辨率化学成像的多元高光谱仪器

• 批准号: 0754740
• 负责人: Dor Ben-Amotz
• 金额: $ 76.12万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2012-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0754740&HistoricalAwards=false
• 关键词: IDBR; Development; Multivariate; Hyper; Spectral

A grant has been awarded to Dr. Ben-Amotz at Purdue University to develop a high speed chemical imaging instrument which will track molecules in living cells. Current chemical imaging instruments are limited by a trade-off between high throughput (speed) and high information content (resolution). This new Multivariate Hyperspectral Imaging (MHI) instrument will overcome these limitations by using a novel data compression strategy to produce a high speed "chemical vision" system. In other words, the MHI instrument will take pictures and movies that reveal where particular molecules are located and how they are moving. In addition to cell imaging, the MHI is expected to be useful for a wide variety of other biological, medical and environmental applications, as further described below. The MHI instrument may be viewed as the first of a new breed of chemical imaging spectrometers which rapidly collects full-spectral images using multivariate data compression. This data compression strategy is related to commonly used music and video data compression methods. However, a key difference is that the MHI uses data compression to speed up the collection of hyperspectral images. This is achieved using a high resolution spatial light modulator (SLM) to rapidly detect full-spectral response functions. The resulting spectral-response intensities may be used either to directly image cells and cell components or to reconstruct complete high resolution spectral images. This hardware data compression strategy is expected to enhance spectral imaging speeds by a factor of 1000, thus facilitating high resolution chemical imaging and dynamics studies of individual live cells, as well as high-throughput/high-content cell screening, and other cell/tissue imaging applications. The MHI instrument is expected to benefit biological and environmental disease/pathogen detection and identification, as well as the education of graduate and undergraduate students. Impacts include fast high-resolution (high-throughput/high-content) imaging of cells for early detection of environmental pathogens and bio-hazards and research leading to elucidation of the mechanisms by which biomolecules influence cellular and tissue function. In other words, the MHI could be used to rapidly read DNA and protein arrays as well as to monitor and map chemicals in environmental and engineering composites. Educational activities include both the specialized training of graduate students in advanced cell handling and imaging techniques, as well as the multi-disciplinary training which graduate and undergraduate students will receive in using MHI instrument for various biological/bio-engineering cell imaging research projects.

普渡大学的Ben-Amotz博士获得了一项拨款，用于开发一种高速化学成像仪器，该仪器将跟踪活细胞中的分子。目前的化学成像仪器受到高通量(速度)和高信息量(分辨率)之间权衡的限制。这种新的多变量高光谱成像(MHI)仪器将通过使用一种新的数据压缩策略来克服这些限制，以产生高速“化学视觉”系统。换句话说，MHI仪器将拍摄照片和电影，揭示特定分子的位置和它们是如何运动的。除了细胞成像，MHI有望用于其他各种生物、医疗和环境应用，如下所述。MHI仪器可以被视为新一代化学成像光谱仪中的第一台，它使用多变量数据压缩来快速收集全光谱图像。该数据压缩策略涉及常用的音乐和视频数据压缩方法。然而，一个关键的区别是，MHI使用数据压缩来加快高光谱图像的收集速度。这是使用高分辨率空间光调制器(SLM)来快速检测全光谱响应函数来实现的。由此产生的光谱响应强度可以用于直接成像细胞和细胞组件，或者用于重建完整的高分辨率光谱图像。这种硬件数据压缩策略有望将光谱成像速度提高1000倍，从而促进单个活细胞的高分辨率化学成像和动力学研究，以及高通量/高含量细胞筛选和其他细胞/组织成像应用。预计MHI仪器将有利于生物和环境疾病/病原体的检测和鉴定，以及研究生和本科生的教育。影响包括对细胞的快速高分辨率(高通量/高含量)成像，以早期检测环境病原体和生物危害，以及有助于阐明生物分子影响细胞和组织功能的机制的研究。换句话说，MHI可以用于快速读取DNA和蛋白质阵列，以及监测和绘制环境和工程复合材料中的化学物质。教育活动包括对研究生进行高级细胞处理和成像技术的专门培训，以及研究生和本科生在使用MHI仪器进行各种生物/生物工程细胞成像研究项目时将接受的多学科培训。