Control of Electrostatic Interactions in Complex Biological Systems
复杂生物系统中静电相互作用的控制
基本信息
- 批准号:0804363
- 负责人:
- 金额:$ 39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-07-01 至 2010-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
ID: MPS/DMR/BMAT(7623) 0804363 PI: Wong, Gerard ORG: University of IllinoisTitle: Control of Electrostatic Interactions in Complex Biological SystemsINTELLECTUAL MERIT: This research program aims to use recently developed knowledge of electrostatics in aqueous media to understand and control complex biological systems governed by a hierarchy of interactions, of which electrostatics is only one part. The aim is to go beyond academic, prototypical electrostatic problems, and engage outstanding unsolved problems in biology. In biological systems, electrostatics coexists with other interactions such as the hydrophobic effect, hydrogen bonding, and geometric/osmotic effects. The proposal addresses three unsolved problems for which such hybrid interactions are essential. First is the formation of complexes of antimicrobial proteins (i.e., lysozyme) with oppositely charged polyelectrolyte (i.e., F-actin) to understand and then control the tendency to form stable complexes. In addition to its potential for biomedical impact, this study is motivated by basic questions about the interactions between periodic charge-densities with different periodicities. Second is a set of X-ray studies of mixtures of model lipid vesicles and pore-inducing TAT (trans-acting activator of transcription) proteins. This section will include studies of the particular role of the positively charged amino acids arginine vs lysine (which seems to go beyond the role of electrostatics to some other mechanism) and include exploratory studies on the ability of TAT to carry cargo across a membrane. The third major activity is a study of synthetic pore-forming antimicrobial compounds synthesized by a collaborator at the University of Massachusetts. X-ray scattering will be used to measure pore structure as a function of concentrations in model membranes and the results will be compared to bacterial response for different variants of the antimicrobial compound.BROADER IMPACTS: Most interactions of biological macromolecules involve forces of several types. The proposal attempts to understand the resultant effect of these hybrid forces in complex systems. The mode of action of membrane pore forming peptides, especially the antimicrobial peptides, is not yet understood. This project seeks fundamental understanding of how naturally occurring and synthetic antimicrobial peptides and other membrane pore formers work. The project provides for highly interdisciplinary training of students and postdoctorals who will gain experience not only in the basic physics and physical chemistry of biological systems but who will also become familiar with the resources of national and international light source facilities. The PI has an excellent record of involving undergraduates in his research program, many of whom are coauthors on publications. He plans to continue to host undergraduate researchers and to give these students the opportunity to experience a research environment that leads many to continue on a research pathway in graduate and professional school. The PI is anxious to expand his outreach activity to the K-12 educational sector beyond the typical "one-shot" university Open House programs and to develop a more enduring relationship with K-12 students and teachers. To this end he has established a partnership with a local middle school, and he will make repeated visits to the school with the goal of "making the strange familiar and the familiar strange" through a series of presentations and activities that give the students and teachers hands-on experience with the properties of interesting materials.
ID: MPS/DMR/BMAT(7623) 0804363 PI: Wong, Gerard ORG:伊利诺伊大学标题:复杂生物系统中静电相互作用的控制知识优点:本研究计划旨在利用最近开发的水介质静电知识来理解和控制由相互作用层次控制的复杂生物系统,其中静电只是其中的一部分。其目的是超越学术的、典型的静电问题,并参与生物学中尚未解决的突出问题。在生物系统中,静电与其他相互作用共存,如疏水效应、氢键和几何/渗透效应。该提案提出了三个尚未解决的问题,这些问题是这种混合相互作用必不可少的。首先是抗菌蛋白(即溶菌酶)与带相反电荷的聚电解质(即f -肌动蛋白)形成复合物,以了解并控制形成稳定复合物的趋势。除了潜在的生物医学影响外,本研究的动机是关于不同周期周期电荷密度之间相互作用的基本问题。其次是一组模型脂质囊泡和诱导孔隙的TAT(反式作用激活因子转录)蛋白混合物的x射线研究。本节将包括对带正电的氨基酸精氨酸和赖氨酸的特殊作用的研究(这似乎超越了静电的作用,达到了一些其他机制),并包括对TAT跨膜运输货物能力的探索性研究。第三项主要活动是由马萨诸塞大学的一位合作者合成的合成成孔抗菌化合物的研究。x射线散射将用于测量孔结构作为模型膜浓度的函数,并将结果与细菌对不同变体的抗菌化合物的反应进行比较。更广泛的影响:大多数生物大分子的相互作用涉及几种类型的力。该提案试图理解这些混合力在复杂系统中的综合效应。膜孔形成肽的作用方式,特别是抗菌肽,尚不清楚。该项目寻求对自然发生和合成的抗菌肽和其他膜孔形成物如何工作的基本理解。该项目为学生和博士后提供高度跨学科的培训,他们不仅将获得生物系统的基础物理和物理化学方面的经验,而且还将熟悉国内和国际光源设施的资源。PI在让本科生参与他的研究项目方面有着出色的记录,其中许多人是出版物的合著者。他计划继续接待本科生研究人员,并让这些学生有机会体验一个研究环境,使许多人继续在研究生和专业学校的研究途径。PI急于将其推广活动扩展到K-12教育部门,而不是典型的“一次性”大学开放日项目,并与K-12学生和教师建立更持久的关系。为此,他与当地一所中学建立了合作关系,他将反复走访学校,通过一系列的演讲和活动,让学生和老师亲身体验有趣材料的特性,“让陌生的熟悉,让熟悉的陌生”。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Gerard Wong其他文献
Surface Sensing, Motility Appendages, and Extracellular Electron Transport in P. Aeruginosa and S. Oneidensis
- DOI:
10.1016/j.bpj.2017.11.199 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Gerard Wong - 通讯作者:
Gerard Wong
Efficient visualization of large routing topologies
大型路由拓扑的高效可视化
- DOI:
10.1145/987185.987189 - 发表时间:
2004 - 期刊:
- 影响因子:1.5
- 作者:
Siew Cheong Au;C. Leckie;A. Parhar;Gerard Wong - 通讯作者:
Gerard Wong
Changes in plasma lipidome following initiation of antiretroviral therapy
开始抗逆转录病毒治疗后血浆脂质组的变化
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:3.7
- 作者:
J. Trevillyan;Gerard Wong;R. Puls;K. Petoumenos;S. Emery;N. Mellett;P. Mundra;P. Meikle;J. Hoy - 通讯作者:
J. Hoy
THU-406 Knowledge, perceptions and barriers to the diagnosis and treatment of hepatitis C among persons who inject drugs: findings from the Educate-Test-Treat (ETT) programme in Singapore
- DOI:
10.1016/s0168-8278(24)02274-8 - 发表时间:
2024-06-01 - 期刊:
- 影响因子:
- 作者:
Alyssa Sim;Shann Long;Sanchalika Acharyya;Gerard Wong;Ryan Jia Jie Liew;Kuo Chao Yew - 通讯作者:
Kuo Chao Yew
Developmental and Intergenerational Landscape of Human Circulatory Lipidome and its Association with Obesity Risk
人类循环脂质组的发育和代际景观及其与肥胖风险的关系
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
S. Mir;Li Chen;S. Burugupalli;B. Burla;S. Ji;A. A. T. Smith;K. Narasimhan;A. Ramasamy;K. Tan;K. Huynh;C. Giles;Ding Mei;Gerard Wong;F. Yap;K. Tan;F. Collier;R. Saffery;P. Vuillermin;A. Bendt;D. Burgner;A. Ponsonby;Y. Lee;Y. Chong;P. Gluckman;J. Eriksson;P. Meikle;M. Wenk;N. Karnani - 通讯作者:
N. Karnani
Gerard Wong的其他文献
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{{ truncateString('Gerard Wong', 18)}}的其他基金
Viral afterlife: Pandemic viruses as rich reservoirs of immunomimetic peptide fragments capable of re-assembly into pro-inflammatory supramolecular complexes
病毒来世:大流行病毒是免疫模拟肽片段的丰富库,能够重新组装成促炎性超分子复合物
- 批准号:
2325840 - 财政年份:2023
- 资助金额:
$ 39万 - 项目类别:
Standard Grant
RAPID: Biomimicry of SARS-CoV-2 and its consequences for infectivity and inflammation
RAPID:SARS-CoV-2 的仿生学及其对感染性和炎症的影响
- 批准号:
2032310 - 财政年份:2020
- 资助金额:
$ 39万 - 项目类别:
Standard Grant
Programming innate immune responses using glycomimetic macromolecular complexes
使用拟糖大分子复合物编程先天免疫反应
- 批准号:
1808459 - 财政年份:2018
- 资助金额:
$ 39万 - 项目类别:
Continuing Grant
Toolbox for hybrid variable-bandwidth bacterio-mimetic antimicrobials
混合可变带宽仿细菌抗菌剂工具箱
- 批准号:
1411329 - 财政年份:2014
- 资助金额:
$ 39万 - 项目类别:
Standard Grant
Molecular-Scale Membrane Curvature Generation in Protein-Lipid Systems: Electrostatics, Hydrophobicity, and Geometry
蛋白质-脂质系统中的分子尺度膜曲率生成:静电、疏水性和几何形状
- 批准号:
1106106 - 财政年份:2011
- 资助金额:
$ 39万 - 项目类别:
Continuing Grant
Integrated Bioengineering Approach to Recovering Antimicrobial Function in Cystic Fibrosis Mucus
恢复囊性纤维化粘液抗菌功能的综合生物工程方法
- 批准号:
1019635 - 财政年份:2009
- 资助金额:
$ 39万 - 项目类别:
Standard Grant
Control of Electrostatic Interactions in Complex Biological Systems
复杂生物系统中静电相互作用的控制
- 批准号:
1019626 - 财政年份:2009
- 资助金额:
$ 39万 - 项目类别:
Continuing Grant
Integrated Bioengineering Approach to Recovering Antimicrobial Function in Cystic Fibrosis Mucus
恢复囊性纤维化粘液抗菌功能的综合生物工程方法
- 批准号:
0827293 - 财政年份:2008
- 资助金额:
$ 39万 - 项目类别:
Standard Grant
Wet Electrostatics and Biomolecular Self-Assembly
湿静电和生物分子自组装
- 批准号:
0409769 - 财政年份:2004
- 资助金额:
$ 39万 - 项目类别:
Continuing Grant
Self-Assembly of Condensed Biomolecular Phases
凝聚生物分子相的自组装
- 批准号:
0071761 - 财政年份:2000
- 资助金额:
$ 39万 - 项目类别:
Continuing Grant
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