Collaborative Research: Biomagnetic Glasses: Preparation, Characterization and Biosensor Applications

Lead ID：DMR/BMAT(7623)0804464 Lead PI：Stanciu，Lia ORG：PurdueNon-Lead ID：DMR/BMAT(7623)0804506非Lead PI：Andreescu，Emanuela ORG：Clarkson标题：协作研究：生物磁玻璃：制备、表征和生物传感器应用INTELLECTUAL优点：本提案的目标是创建一种用于制造具有磁能力的生物功能材料的通用方法，即所谓的“生物磁玻璃”。这些将用于设计高度稳定、可磁分离的基于酶的系统。该项目的重点是创造含有酶、磁性纳米颗粒和二氧化硅玻璃的混合复合材料，并开发将用于制备这些材料的通用策略。三种酶被选为模型例子：辣根过氧化物酶(HRP)、乙酰胆碱酯酶(AChE)和葡萄糖-6-磷酸脱氢酶(G6PDH)。作为拟议应用的一个例子，基于AChE的生物磁性玻璃将适合用作传感部件，用于开发廉价且自主操作的生物分析设备，用于检测食品、农业领域和其他安全情况下的农药。有人提出，传感器材料的磁性将允许它们使用永久磁体相对于潜在分析物进行局部化。该项目的具体目标是：(1)合成和表征具有可控微结构和性能的二氧化硅-铁氧化物磁性纳米颗粒。(2)将模型酶(HRP、AChE、G6PDH)包裹在二氧化硅磁性纳米复合材料中，并监测其生物催化活性和稳定性(普渡)；(3)使用丝网印刷电极开发生物传感器中的生物磁性玻璃(Clarkson)；(4)在应用不同浓度的目标分析物(如过氧化氢、杀虫剂、葡萄糖-6-磷酸)时，建立生物传感器的校准曲线图(Clarkson)。该提案特别涉及基于酶的生物传感器的使用寿命问题。该项目为研究生和本科生的多学科培养提供了一个极好的平台。这两个私人投资机构都鼓励本科生参与；克拉克森的预算中包括支持本科生的资金。这位总部位于普渡的PI计划将生物传感器的材料纳入她在她所在的两所学校任教的课程中。这两个私人助理都将与当地公立学校进行接触。在克拉克森，这些关系已经建立并持续存在，而普渡大学的PI正在与学校系统发展必要的关系。她还积极参加普渡大学的女性工程项目，并成功地招募了女性学生加入她的研究小组。这两家个人投资机构都表现出对扩大代表不足的少数族裔学生参与的敏感性。私人投资机构与各自校园正在进行的少数族裔招聘计划有关。

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: PurdueNon-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: ClarksonTitle: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor ApplicationsINTELLECTUAL MERIT: The goal of this proposal is to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities, so called "biomagnetic glasses." These will be used in the design of highly stable, magnetically separable enzyme-based systems. The project focuses on creating hybrid composite materials containing enzymes, magnetic nanoparticles, and silica glasses and on the development of generic strategies that will be used to prepare these materials. Three enzymes have been selected as model examples: horseradish peroxidase (HRP), acetylcholinesterase (AChE), and glucose-6-phosphate dehydrogenase (G6PDH). As an example of proposed applications, the AChE based biomagnetic glasses will be suitable for use as the sensing component in the development of a cheap and autonomously operating bioanalytical device for detecting pesticides in food products, agricultural fields and other security situations. It is proposed that the magnetic properties of the sensor materials will permit them to be localized with respect to the potential analytes using permanent magnets. The specific objectives of the project are: (1) synthesize and characterize silica-iron oxide magnetic nanoparticles with controlled microstructure and properties. (Purdue); (2) encapsulate model enzymes (HRP, AChE, G6PDH) within the silica magnetic nanocomposites and monitor the biocatalytic activity and stability (Purdue); (3) use screen printed electrodes to exploit the biomagnetic glasses in biosensors (Clarkson); (4) build calibration plots of the biosensors upon application of various concentrations of target analytes (e.g. hydrogen peroxide, pesticides, glucose-6-phosphate) (Clarkson).BROADER IMPACTS: Bio-based sensor technology that is sensitive and selective across a broad dynamic range remains in great demand for analysis of chemical species in biotech process streams, in the environment, and in the security area. This proposal addresses, in particular, the issue of useful lifetime of enzyme-based biosensors. The project provides an excellent platform for multidisciplinary training of graduate and undergraduate students. Both PIs encourage undergraduate participation; funding for support of undergraduates is included in the Clarkson budget. The Purdue-based PI plans to incorporate materials on biosensors in courses that she teaches in the two Schools of which she is a member. Both PIs will engage in outreach to local public schools. At Clarkson these relationships are established and on-going, while the PI at Purdue is developing the necessary relationships with the school system. She is also active in the Purdue Women in Engineering program and has been successful in recruiting female students into her research group. Both PIs demonstrate a sensitivity toward broadening participation among underrepresented minority students. The PIs are connected with ongoing minority recruitment programs on their respective campuses.