Telechelic polymers by RAFT polymerization and application to stable bFGF-polymer conjugates

通过 RAFT 聚合制备遥爪聚合物并应用于稳定的 bFGF-聚合物缀合物

• 批准号: 0809832
• 负责人: Heather Maynard
• 金额: $ 42.3万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0809832&HistoricalAwards=false
• 关键词: Telechelic; polymers; RAFT; polymerization; application

This project will continue work on the use of controlled radical polymerizations to synthesize well-defined protein-polymer conjugates. Many proteins exist as multimers in the active state, yet most conjugates contain only one protein due to the lack of high yielding, efficient synthetic strategies to make the polymers. In this research, a straightforward method to synthesize biocompatible polymers with protein-reactive groups at one or both ends will be developed. The polymers will then be applied to produce conjugates of a growth factor that is critical in wound healing. Because this growth factor exists as a dimer in the active state, the growth factor conjugate is expected to be more bioactive. Conjugates containing polymers that mimic polysaccharides in the body will also be prepared to form growth factors resistant to denaturation upon application and storage. With this award, the Organic and Macromolecular Chemistry Program is supporting the research of Professor Heather D. Maynard of the Department of Chemistry and Biochemistry at the University of California, Los Angeles. Professor Maynard's research focuses on the synthesis of well-defined multimeric protein-polymer conjugates by controlled radical polymerizations. This work will contribute to the synthesis of active and stable conjugates for therapeutic applications. Successful development of the methodology will have an impact on the polymer, biomedical and materials communities.

本项目将继续致力于利用可控自由基聚合来合成定义明确的蛋白质-聚合物偶联物。许多蛋白质在活性状态下作为多聚体存在，但由于缺乏高产、高效的合成策略来制造聚合物，大多数缀合物只含有一种蛋白质。在本研究中，将开发一种直接合成一端或两端具有蛋白质反应基团的生物相容性聚合物的方法。然后，聚合物将被用于生产一种生长因子的共轭物，这种生长因子对伤口愈合至关重要。由于该生长因子以二聚体的形式存在于活性状态，因此该生长因子偶联物有望具有更高的生物活性。含有模拟体内多糖的聚合物的缀合物也将被制备成在应用和储存时形成抗变性的生长因子。通过这个奖项，有机和大分子化学项目支持了加州大学洛杉矶分校化学和生物化学系Heather D. Maynard教授的研究。梅纳德教授的研究重点是通过控制自由基聚合合成定义明确的多聚体蛋白质-聚合物缀合物。这项工作将有助于合成用于治疗的活性和稳定的偶联物。该方法的成功开发将对聚合物、生物医学和材料界产生影响。