SBIR Phase I: A Label-Free SERS-Capture Assay in Microchips for Biological Warfare Agents

SBIR 第一阶段：用于生物战剂的微芯片中的无标记 SERS 捕获分析

• 批准号: 0810335
• 负责人: Frank Inscore
• 金额: $ 10万
• 依托单位: REAL-TIME ANALYZERS, INCORPORATED
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0810335&HistoricalAwards=false
• 关键词: SBIR; Phase; Label; Free; SERS

This Small Business Innovation Research Phase I project develops assay chips that can detect, identify, and quantify the presence of Category A or B bioagents at the required sensitivity within 10 minutes. The assay chips will employ silver particles in a porous sol-gel to selectively extract and bind a specific bioagent with detection using surface-enhanced Raman spectroscopy (SERS). The chips will be part of an analyzer that also includes an aerosol collector and a field usable Raman spectrometer. Feasibility of the approach will be demonstrated during Phase I by developing the silver functionalization method to bind Bacillus cereus and Bacillus subtilis spores, and to detect the capture event by SERS. During the Phase II project, assay chips will be developed and field-tested for several Category A or B bioagents.The broader impacts of this research an enchancement of our ability to monitor public places targeted by terrorists, and to implement rapid response and countermeasures. This information will save lives and substantially reduce the terror associated with such attacks. The device can also be adapted to detect more common Category B pathogens regarding food and water safety (e.g. E. coli 0157:H7).

该小型企业创新研究第一阶段项目开发的检测芯片可以在 10 分钟内以所需的灵敏度检测、识别和量化 A 类或 B 类生物制剂的存在。 该检测芯片将采用多孔溶胶-凝胶中的银颗粒来选择性地提取和结合特定的生物剂，并使用表面增强拉曼光谱（SERS）进行检测。 这些芯片将成为分析仪的一部分，该分析仪还包括气溶胶收集器和现场可用的拉曼光谱仪。 该方法的可行性将在第一阶段通过开发银功能化方法来结合蜡样芽孢杆菌和枯草芽孢杆菌孢子并通过 SERS 检测捕获事件来证明。 在第二阶段项目中，将针对多种 A 类或 B 类生物制剂开发检测芯片并进行现场测试。这项研究的更广泛影响是提高我们监测恐怖分子瞄准的公共场所以及实施快速反应和对策的能力。 这些信息将拯救生命并大大减少与此类袭击相关的恐怖。 该设备还可用于检测与食品和水安全有关的更常见的 B 类病原体（例如大肠杆菌 0157:H7）。