SBIR Phase I: A Platform for Label-Free, Numerous-Marker-Based Isolation of Functionally Preserved Single Cancer Stem Cells

这个SBIR第一阶段项目的更广泛的影响/商业潜力利用了节点-孔道检测和多标记筛选，用于可行的、功能性保存的单个膀胱干细胞尿路上皮癌的分离。这一工具将有助于更全面地发现生物标记物，并促进靶向治疗的发展。最终，这一解决方案将允许临床、学术和工业研究人员有选择地从脆弱的初级膀胱活检亚群中分离单个细胞，用于下游分析和长期的个性化治疗开发。能够以一种简单、可用和低成本的方式筛选和分离膀胱干细胞的靶向尿路上皮癌，这将使下游功能研究能够帮助改善患者的预后。结合这里的商业机会，通过将低成本的膀胱上皮癌干细胞分离仪器引入研究、工业/生物制药和临床设施，预计将对研究产出和出版物产生重大的积极影响。这个SBIR第一阶段项目涉及一种多参数方法，利用无标记的结孔传感和低剪切微流控技术进行可行的单细胞分选和分配，同时保持下游分析的活性和功能特性。膀胱癌干细胞的单个活体功能保存的尿路上皮癌的鉴定和分离对于确定被普遍接受的、临床上准确的早期癌症检测生物标志物以及优化靶向治疗开发至关重要。该项目的目的是在不需要大量实验设计和调整的情况下提供对这些单元的轻松访问，使未经培训的非专家用户能够进行操作。使用新的检测和分离方法，该平台可用于揭示被掩盖的异质性，并使单独分离的、可存活的和功能性保留的膀胱干细胞孤立的尿路上皮癌的下游功能研究(如生长、侵袭和化疗耐药性)成为可能。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this SBIR Phase I project leverages node-pore sensing with multi-marker screening for viable, functionally-preserved isolation of single urothelial carcinoma of the bladder stem cells. This tool will help enable more comprehensive biomarker discovery and facilitate the development of targeted therapies. Ultimately, this solution will allow for clinical, academic, and industrial researchers to selectively isolate single cells from fragile primary bladder biopsy subpopulations for downstream analysis and personalized treatment development in the longer term. The ability to screen for numerous parameters and isolate target urothelial carcinoma of the bladder stem cells in a walk-up usable, accessible, and low-cost manner will enable downstream functional studies that will help lead to improved patient outcomes. In conjunction with the business opportunity here, there is projected to be a significant positive impact on research output and publications that will be enabled by the introduction of a low-cost urothelial carcinoma of the bladder stem cell isolation instrument to research, industrial/biopharma, and clinical facilities.This SBIR Phase I project involves a multi-parametric approach leveraging label-free node-pore sensing and low-shear microfluidics for viable single-cell sorting and dispensing while preserving viability and functional characteristics for downstream assays. Characterization and isolation of single, live functionally-preserved urothelial carcinoma of the bladder cancer stem cells is pivotal for the determination of universally accepted, clinically-accurate biomarkers for early cancer detection as well as optimized targeted therapy development. This project is aimed at delivering easy access to these cells without requiring extensive experimental design and tuning, enabling operation by untrained, non-expert users. Using the novel detection and isolation methodologies, the platform can be used to unveil masked heterogeneity and enable downstream functional studies (such as growth, invasion, and chemotherapeutic resistance) on individually isolated, viable, and functionally-preserved isolated urothelial carcinoma of the bladder stem cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.