SBIR Phase II: A Label-Free Surface-enhanced Raman Spectroscopy-Capture Assay in Microchips for Biological Warfare Agents

SBIR 第二阶段：用于生物战制剂的微芯片中的无标记表面增强拉曼光谱捕获分析

• 批准号: 0956170
• 负责人: Frank Inscore
• 金额: $ 49.39万
• 依托单位: REAL-TIME ANALYZERS, INCORPORATED
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0956170&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Label; Free

This Small Business Innovation Research (SBIR) Phase II project will develop a prototype analyzer that can detect, identify, and quantify the presence of Category A (B and C) bioagents at the required specificity and sensitivity (e.g. in air 104 spores/m3, 100 organisms/m3 and 2-300 mg toxin/m3) within 10 minutes. The analyzers will incorporate a novel surface-enhanced Raman spectroscopy (SERS)-based assay into sample systems read by a portable Raman spectrometer. The assays will be functionalized to selectively capture specific bioagents and generate unique SER spectra when irradiated by the analyzer laser. During Phase I, feasibility was demonstrated by selectively binding and detecting 25 ppm B. cereus (a B. anthracis surrogate) in the presence of 250 ppm B. subtilis. During Phase II, the assays will be developed to detect several real agents, such as B. anthracis (anthrax), Yersinia pestis (Plague), Francisella tularensis (Tularemia), and Clostridium botulinum (Botulism). The assays will then be incorporated into a product prototype that autonomously detects aerosolized bioagents. The analytical capabilities of the prototype will be validated at the US Army's Edgewood Chemical Biological Center.The broader/commercial impact of this project will provide a bioagent detector with the necessary sensitivity and speed to save lives and reduce substantially the terror associated with biological attacks. The continued presence of US military personnel in the Middle East has produced a persistent fear that biological warfare agents may be used by terrorists against civilian and military personnel at home and abroad. The proposed analyzer will allow measurement of such bioagents within 10 minutes, a vast improvement over the 2-3 hours required by current technology. Initially, the proposed detector will be used to protect military bases. Once established, the application of this product will be expanded to civilian applications, such as transit systems, high profile buildings (federal, financial, Fortune 500), stadiums, airports, and malls. The US military and civilian market is currently estimated at $0.5 Billion.

该小型企业创新研究（SBIR）第二阶段项目将开发一种原型分析仪，该分析仪可以在10分钟内以所需的特异性和灵敏度（例如，在空气中104个孢子/m3，100个微生物/m3和2-300 mg毒素/m3）检测，识别和量化A类（B和C）生物制剂的存在。 该分析仪将采用一种新的表面增强拉曼光谱（Sers）为基础的分析到样品系统读取的便携式拉曼光谱仪。检测将被功能化，以选择性地捕获特定的生物制剂，并在分析仪激光照射时产生独特的SER光谱。在阶段I期间，通过选择性结合和检测25 ppm B证明了可行性。蜡状（a B. anthracis替代物）在250 ppm B.枯草杆菌。在第II阶段，将开发检测几种真实的病原体（如B）的检测方法。炭疽（炭疽）、鼠疫耶尔森氏菌（鼠疫）、土拉热弗朗西丝氏菌（土拉菌病）和肉毒梭菌（肉毒杆菌中毒）。然后，这些检测方法将被纳入一个产品原型，自动检测气溶胶化的生物制剂。该原型的分析能力将在美国陆军埃奇伍德化学生物中心得到验证，该项目的广泛/商业影响将提供一种具有必要灵敏度和速度的生物制剂探测器，以拯救生命，并大大减少与生物袭击有关的恐怖。美国军事人员在中东的持续存在使人们一直担心，恐怖分子可能会对国内外的平民和军事人员使用生物战剂。拟议的分析仪将允许在10分钟内测量此类生物制剂，这比当前技术所需的2-3小时有了巨大的改进。最初，拟议中的探测器将用于保护军事基地。一旦建立，该产品的应用将扩展到民用应用，如交通系统，高姿态建筑（联邦，金融，财富500强），体育场，机场和商场。美国军用和民用市场目前估计为5亿美元。