Enhancing Research and Education at the University of Alaska Anchorage with a state-of-the-art Liquid Chromatograph-Ion Trap Mass Spectrometer

获得重大研究仪器计划(MRI)的这一奖项后，来自阿拉斯加大学安克雷奇(UAA)校区的Birgit Hagedorn及其同事Marc C.Perry、Donald E.Spallinger和Jeffrey M.Welker将获得与串联质谱仪(LC/MS/MS)相耦合的高效液相色谱。质谱学提供了强大的基于各种碎片质量的化合物鉴定和定量，以及用于确定复杂混合物的组分的分子量，例如脂类、持久性有机污染物和单宁，这些都是UAA教职员工和学生目前的研究课题。此外，质谱仪将与离子色谱仪(IC)相结合，以分离复杂基质中低浓度的有毒高氯酸盐等阴离子，这是UAA的另一个活跃研究课题。该光谱仪是理想的，因为它提供了不同的电离模式，在宽质量范围内(m/z 50-2200)灵敏度为250毫微克。该仪器将设在UAA的应用科学、工程和技术(ASET)实验室，实验室经理和化学家将提供所需的知识和技能，以建立方法，维护该仪器的性能，并培训教师和学生。质谱学(MS)是一种用于探测亲密结构细节和获得大量有机、生物有机和有机金属分子的分子组成的技术。它是发展最快、使用最广泛的分析仪器技术之一。正因为如此，让本科生和硕士研究生接触到这项技术是很重要的。大致来说，串联质谱计可以被认为是两个串联的质谱计，由一个可以将分子分解成碎片的腔室连接。

With this award from the Major Research Instrumentation program (MRI), Birgit Hagedorn and colleagues Marc C. Perry, Donald E. Spallinger and Jeffrey M. Welker from the University of Alaska Anchorage (UAA) Campus will acquire high performance liquid chromatography coupled to a tandem mass spectrometer (LC/MS/MS). Mass spectrometry provides powerful compound identification and quantification based on the masses of various fragments as well as for determining the molecular weight of the components of complex mixtures such as lipids, persistent organic pollutants, and tannins which are currently research topics of UAA faculty and students. Furthermore, the mass spectrometer will be combined with an ion chromatograph (IC) to separate anions, such as toxic perchlorate at low concentration in complex matrixes, which is another active research topic at UAA. The spectrometer is ideal because it provides different modes of ionization with a sensitivity of 250 femtograms over a wide mass range (m/z 50-2200). The instrument will be hosted in the Applied Science, Engineering, and Technology (ASET) Laboratory at UAA where a laboratory manager and chemist will provide the knowledge and skills needed to establish methods, maintain the performance of this instrument, and train faculty and students.Mass spectrometry (MS) is a technique used to probe intimate structural details and to obtain the molecular compositions of a vast array of organic, bioorganic, and organometallic molecules. It is one of the fastest growing and most widely used analytical instrumentation techniques. Because of this, it is important for undergraduate and master students to be exposed to the technique. To a first approximation a tandem mass spectrometer can be thought of as two mass spectrometers in series connected by a chamber that can break a molecule into pieces.