SGER: Linking enzymes to light emitting semiconductors for high throughput screening

SGER：将酶与发光半导体连接以进行高通量筛选

• 批准号: 0814646
• 负责人: Daniel Bond
• 金额: --
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0814646&HistoricalAwards=false
• 关键词: SGER; Linking; enzymes; light; emitting

CBET-0814646BondHighly efficient enzyme-functionalized electrodes are essential for enzymatic fuel cells, electrobiocatalysis and other renewable energy applications. There is currently no available electrochemical technology enabling high throughput selection and improvement of electrode bound enzymes, due to limits in highly parallel sensing of very low rates of electron flow. The central goal of this project is to demonstrate a solution to this issue: a micro-array of enzyme-functionalized light emitting semiconductor based electrodes. By fabricating LED micro-arrays, high throughput screening of electricity producing enzymes and bacteria via light detection will become possible. Future versions of this approach will aim to allow on-chip PCR amplification and in vitro translation of protein libraries, eliminating a key bottleneck (cloning and purification) in enzyme discovery. Intellectual Merit: Combining enzymes and semiconductors for electron transfer-dependent light generation and detection is a novel approach. This interdisciplinary project combines the knowledge of protein chemistry and biology with electronics and electrical engineering. Broader impact: Enzyme-LED micro-arrays would have an impact on the pace of biocatalyst development for green biotechnology and bio-fuel cells utilizing renewable carbon sources with improved efficiency. This novel approach could resolve a crucial limitation in development of enzyme functionalized electrodes, and will represent cross-disciplinary training for a team of chemistry, biochemistry, and engineering students.

CBET-0814646 Bond高效酶功能化电极对于酶燃料电池、电生物催化和其他可再生能源应用至关重要。由于在非常低的电子流速率的高度并行感测中的限制，目前没有能够实现高通量选择和电极结合酶的改进的可用电化学技术。该项目的中心目标是展示这个问题的解决方案：基于酶功能化发光半导体电极的微阵列。通过制造LED微阵列，通过光检测高通量筛选产电酶和细菌将成为可能。这种方法的未来版本将旨在允许芯片上PCR扩增和蛋白质库的体外翻译，消除酶发现中的关键瓶颈（克隆和纯化）。智力优势：将酶和半导体结合用于电子转移依赖的光产生和检测是一种新方法。这个跨学科的项目结合了蛋白质化学和生物学与电子和电气工程的知识。更广泛的影响：酶发光二极管微阵列将对利用可再生碳源的绿色生物技术和生物燃料电池的生物催化剂开发速度产生影响，并提高效率。这种新方法可以解决酶功能化电极开发中的一个关键限制，并将代表化学，生物化学和工程专业学生团队的跨学科培训。