Characterization of myeloid suppressor cell activity in experimental leishmaniasis

实验性利什曼病骨髓抑制细胞活性的表征

• 批准号: 188763720
• 负责人: Professor Dr. Uwe Ritter
• 金额: --
• 依托单位: Institut für Immunologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/188763720?language=en
• 关键词: Characterization; myeloid; suppressor; cell; activity

In experimental leishmaniasis, the clinical course of disease depends on the genetically determined ability of the infected mouse strain to mount a protective T cell-mediated immune response. C57BL/6 mice control parasite replication, whereas BALB/c mice develop a chronic course of disease. Considering these divergent phenotypes, it is most likely that parameters regulating the adaptive immune response against L. major parasites differ in C57BL/6 and BALB/c mice. Recently, myeloid suppressor cells have been described to suppress T cell functions. The most potent suppressive cells are inflammatory CD11b+ monocytes (IMC) that are characterized by the expression of high levels of lymphocyte antigen 6 complex C (Ly-6C), but are negative for Ly-6G. We quantified IMCs at the site of infection and skin-draining lymph nodes (SDLNs) of infected C57BL/6 and BALB/c mice. BALB/c mice showed an increase of IMC at the site of infection and SDLNs, whereas the numbers of IMCs in C57BL/6 SDLNs were not affected. Thus, we propose the hypothesis that IMCs migrate to the site of infection and SDLNs of BALB/c mice to suppress the development of an efficient T cell response. The overall goal of this project is to characterize the different T cell-suppressing mechanisms mediated by IMCs in the experimental model of leishmaniasis.

在实验性利什曼病中，疾病的临床病程取决于受感染小鼠品系的基因决定能力，该品系能够发起保护性T细胞介导的免疫反应。C57BL/6小鼠控制寄生虫复制，而BALB/c小鼠则发展为慢性病程。考虑到这些不同的表型，C57BL/6和BALB/c小鼠调节对L. major寄生虫适应性免疫反应的参数很可能是不同的。最近，骨髓抑制细胞被描述为抑制T细胞功能。最有效的抑制细胞是炎性CD11b+单核细胞（IMC），其特征是高水平表达淋巴细胞抗原6复合物C (Ly-6C)，但对Ly-6G呈阴性。我们量化了感染C57BL/6和BALB/c小鼠感染部位和皮肤引流淋巴结（SDLNs）的IMCs。BALB/c小鼠感染部位的IMC和SDLNs增加，而C57BL/6 SDLNs的IMCs数量不受影响。因此，我们提出假设IMCs迁移到BALB/c小鼠的感染部位和SDLNs，以抑制有效T细胞反应的发展。本项目的总体目标是在利什曼病实验模型中表征由IMCs介导的不同T细胞抑制机制。

项目成果

An Emerging Approach for Parallel Quantification of Intracellular Protozoan Parasites and Host Cell Characterization Using TissueFAXS Cytometry

• DOI: 10.1371/journal.pone.0139866
• 发表时间: 2015-10-21
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Schmid, Maximilian;Dufner, Bianca;Ritter, Uwe
• 通讯作者: Ritter, Uwe