COLLABORATIVE RESEARCH: Evolution of Sex Chromosomes in Turtles

合作研究：海龟性染色体的进化

• 批准号: 0817687
• 负责人: Scott Edwards
• 金额: $ 20.5万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0817687&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; Evolution; Sex; Chromosomes

Sex is determined by genetic constitution (GSD) in most animals, such as by the sex chromosome pairs XX or XY in humans, or ZZ or ZW in birds. In certain other animals, however, sex is determined by environmental temperature (TSD). Among turtle species, however, examples of both types of sex determination can be found. It remains unclear, however, how and why different sex determining mechanisms exist. This collaborative project will help fill this gap by comparing the chromosomes and sex-related genes of turtles using XX/XY, ZZ/ZW, or TSD mechanisms for sex determination. Particularly, this research will test whether (1) all sex chromosomes in turtles derive from a common ancestral pair of non-sex chromosomes (or "autosomes"), and whether (2) the molecular evolution of genes located on turtle sex chromosomes differs from that of the same genes in TSD turtles, in which there are no sex chromosomes- i.e., whether the sequences of genes located on sex chromosomes change differently over evolutionary time than do the sequences of the same genes when they are located on autosomes. This project will integrate undergraduate discovery-based learning for undergraduates into the field and lab modules of this project and will broaden the participation of underrepresented groups in biology by providing direct training opportunities for students and their mentors, by engaging women and minorities through outreach activities, and by providing further opportunities for both the investigators to serve as role models for minorities.

在大多数动物中，性别是由遗传构成（GSD）决定的，例如人类的性染色体对XX或XY，鸟类的ZZ或ZW。然而，在某些其他动物中，性别取决于环境温度（TSD）。然而，在海龟物种中，可以找到这两种性别决定的例子。然而，仍然不清楚如何以及为什么存在不同的性别决定机制。这个合作项目将有助于填补这一空白，通过比较染色体和性别相关基因的海龟使用XX/XY，ZZ/ZW，或TSD机制的性别决定。特别是，这项研究将测试（1）海龟中的所有性染色体是否都来自一对共同的祖先非性染色体（或“常染色体”），以及（2）位于海龟性染色体上的基因的分子进化是否不同与TSD海龟中相同基因的分子进化不同，其中没有性染色体--即，位于性染色体上的基因序列在进化过程中的变化是否与位于常染色体上的相同基因序列不同。该项目将把本科生基于发现的学习纳入该项目的实地和实验室模块，并将通过为学生及其导师提供直接培训机会，通过外联活动吸引妇女和少数群体参与，以及为调查人员提供更多机会，使他们成为少数群体的榜样，扩大代表性不足群体对生物学的参与。