Collaborative Research: CPSF30 at the Convergence of RNA Processing, Cellular Signaling, and Development in Plants

亚瑟G.亨特和Q。Quinn Li IOS-0817818合作提案：CPSF 30在RNA加工、细胞信号传导和植物发育中的融合基因表达在许多水平上受到控制，包括RNA的加工。 关于环境输入（刺激）导致RNA加工变化的机制仍然存在许多问题。 该项目的研究涉及一种可能的机制，涉及一种RNA加工因子（CPSF 30）在植物基因表达调控中的作用。这项工作的灵感来自于先前的观察，即不产生蛋白质的突变植物具有一系列有趣的表型，并且蛋白质本身受细胞感觉系统的中心成分调节。 该项目将结合联合收割机遗传学，生物化学，分子和系统的方法来了解细胞信号和CPSF 30的功能之间的相互作用。 该项目的研究部分的结果将是更好地了解刺激可以引起植物细胞中RNA加工变化的方式。 这反过来将有助于更好地了解植物对环境线索和致病生物的反应方式。 该项目的影响将远远超出对植物基因表达的理解。 预期的结果将有助于开发新的遗传和生物技术策略，以改善作物在不利条件下的表现。 该项目将涉及博士后科学家，研究生和本科生。有一个决定跨学科的性质，这个项目，因为它涉及分子/生物化学，整株植物和系统生物学的方法。因此，这些研究将有助于培训学员在一个日益跨学科的科学领域（植物生物学）作出贡献。 参加者（包括PI）将参与社区计划，以促进公众对科学的兴趣和欣赏。

Arthur G. Hunt and Q. Quinn Li IOS-0817818Collaborative Proposal: CPSF30 at the convergence of RNA processing, cellular signaling, and development in plantsGene expression is controlled at many levels, including the processing of RNAs. There remain many questions as to the mechanisms by which environmental inputs (stimuli) lead to changes in RNA processing. The research to be pursued in this project deals with one possible mechanism, involving the role of one RNA processing factor (CPSF30) in regulated gene expression in plants. This work is inspired by prior observations that mutant plants that do not make the protein have an interesting range of phenotypes, and that the protein itself is regulated by a central component of cellular sensory systems. This project will combine genetic, biochemical, molecular, and systems approaches to understand the interplay between cellular signaling and the function of CPSF30. The outcome of the research component of the project will be a better understanding of the ways in which stimuli can cause changes in the processing of RNAs in the plant cell. This will in turn contribute to a better understanding of the ways by which plants respond to environmental cues and disease-causing organisms. This project will have an impact that extends significantly beyond an understanding of gene expression in plants. The expected results will be of help in developing new genetic and biotechnological strategies for improving the performance of crop plants in adverse conditions. This project will involve postdoctoral scientists, graduate students, and undergraduate students. There is a decided cross-disciplinary nature to this project, in that it involves molecular/biochemical, whole-plant, and systems biology approaches. As such, these studies will help to prepare trainees to make contributions in a scientific field (plant biology) that is becoming more cross-disciplinary. Participants (including PIs) will participate in community programs so as to promote interest in and appreciation of science in the general public.