Arabidopsis 2010: Arabidopsis Polyadenylation Factor Subunits - Mutants and Protein Interaction Networks

信使RNAs的多糖基化是植物基因表达过程中的一个重要步骤。多聚腺苷酸化为信使核糖核酸的合成增加了一种必不可少的元素，并有助于协调信使核糖核酸的合成，最终发挥其在蛋白质合成中的作用。因此，组成聚腺苷酸化机制的蛋白质亚基有望在植物生长中发挥重要作用。这个项目的目标是确定编码多聚腺苷化机制亚单位的一组拟南芥基因的集体功能(这一组基因，包括拟南芥基因组标识符，可以在http://www.uky.edu/~aghunt00/polyA2010.html和http://www.users.muohio.edu/liq/arabidopsis2010.html).上找到这将包括一个或多个亚基被移除(通过突变或基因沉默)的植物的表型文件，以及涉及各种亚基的一整套蛋白质-蛋白质相互作用的确定。结果将通过同行评议的出版物以及主要调查人员管理的网页(http://www.uky.edu/~aghunt00/polyA2010.html和http://www.users.muohio.edu/liq/arabidopsis2010.html).)传播此外，突变的拟南芥库存将存放在适当的库存中心(S)。这项研究将通过对突变表型的分类，通过定义拟南芥多腺化因子亚单位之间的相互作用网络，并通过识别与多腺苷化机制相互作用的未知功能的蛋白质，为2010年计划的目标(到2010年确定参考物种的所有基因在其细胞、生物和进化背景下的功能)做出贡献。这项研究将极大地提高目前对高等植物中mRNA3‘末端形成的理解。因此，它将提高定制外源基因在转基因植物中表达的能力，从而为农民开发改良作物(以及新的替代经济机会)。这也将有助于深入了解信使核糖核酸多腺苷的进化过程。研究产品(克隆的基因、抗体、植物品系等)将向广大研究社区提供。这项研究将有助于培养两名博士后学者、四名研究生和多达十二名本科生。后者将从肯塔基大学和迈阿密大学备受推崇的多样化课程中挑选出来。

The polyadenylation of messenger RNAs (mRNAs) is an important step in the process of gene expression in plants. Polyadenylation adds an essential element to the mRNA and helps to coordinate the synthesis of a messenger RNA with its ultimate functioning in protein synthesis. Consequently, the protein subunits that make up the polyadenylation machinery are expected to play important roles in plant growth. The goals of this project are to determine the collective function of the set of Arabidopsis genes that encode subunits of the polyadenylation machinery (this set, including Arabidopsis genome identifiers, can be found at http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). This will include a documentation of the phenotypes of plants that have had one or more subunit removed (by mutation or gene silencing) and a determination of the complete set of protein-protein interactions involving the various subunits. The results will be disseminated via peer-reviewed publication as well as through web pages managed by the principal investigators (http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). In addition, mutant Arabidopsis stocks will be deposited with the appropriate stock center(s). The research will contribute to a goal of the 2010 Project ("to determine the function of all genes of a reference species within their cellular, organismal and evolutionary contexts by the year 2010") by cataloguing mutant phenotypes, by defining the network of interactions amongst Arabidopsis polyadenylation factor subunits, and by identifying proteins of unknown function that interact with the polyadenylation machinery.The research will greatly increase the current understanding of mRNA 3' end formation in higher plants. As such, it will enhance the ability to tailor foreign genes for expression in transgenic plants, and thereby develop improved crops (as well as novel alternative economic opportunities) for farmers. It will also lend significant insight into the evolution of mRNA polyadenylation. The research products (cloned cDNA, antibodies, plant lines etc.) will be made available to the research community at large. The research will contribute to the training of two postdoctoral scholars, four graduate students, and as many as twelve undergraduates. The latter will be drawn from highly-regarded and diverse programs at the University of Kentucky and Miami University.