Arabidopsis 2010: Arabidopsis Polyadenylation Factor Subunits - Mutants and Protein Interaction Networks

信使RNA (mRNA) 的多聚腺苷酸化是植物基因表达过程中的重要步骤。 多聚腺苷酸化为 mRNA 添加了必需元素，有助于协调信使 RNA 的合成及其在蛋白质合成中的最终功能。 因此，构成多聚腺苷酸化机制的蛋白质亚基预计将在植物生长中发挥重要作用。 该项目的目标是确定编码多聚腺苷酸化机制亚基的拟南芥基因组的集体功能（该组基因，包括拟南芥基因组标识符，可以在http://www.uky.edu/~aghunt00/polyA2010.html和http://www.users.muohio.edu/liq/arabidopsis2010.html找到）。 这将包括已删除一个或多个亚基（通过突变或基因沉默）的植物表型的记录，以及涉及各个亚基的全套蛋白质-蛋白质相互作用的确定。 结果将通过同行评审出版物以及主要研究人员管理的网页（http://www.uky.edu/~aghunt00/polyA2010.html 和 http://www.users.muohio.edu/liq/arabidopsis2010.html）进行传播。 此外，突变拟南芥种群将存放在适当的种群中心。该研究将通过对突变表型进行编目、定义拟南芥聚腺苷酸化因子亚基之间的相互作用网络以及识别与聚腺苷酸化机制相互作用的未知功能的蛋白质，为2010年项目的目标（“到2010年确定参考物种的所有基因在其细胞、有机体和进化环境中的功能”）做出贡献。该研究将极大地增进目前对高等植物中mRNA 3'末端形成的理解。 因此，它将增强定制外源基因在转基因植物中表达的能力，从而为农民开发改良作物（以及新的替代经济机会）。 它还将为 mRNA 多腺苷酸化的进化提供重要的见解。 研究产品（克隆的 cDNA、抗体、植物系等）将提供给整个研究界。 该研究将有助于培养两名博士后学者、四名研究生和多达十二名本科生。 后者将来自肯塔基大学和迈阿密大学备受推崇的多元化项目。

The polyadenylation of messenger RNAs (mRNAs) is an important step in the process of gene expression in plants. Polyadenylation adds an essential element to the mRNA and helps to coordinate the synthesis of a messenger RNA with its ultimate functioning in protein synthesis. Consequently, the protein subunits that make up the polyadenylation machinery are expected to play important roles in plant growth. The goals of this project are to determine the collective function of the set of Arabidopsis genes that encode subunits of the polyadenylation machinery (this set, including Arabidopsis genome identifiers, can be found at http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). This will include a documentation of the phenotypes of plants that have had one or more subunit removed (by mutation or gene silencing) and a determination of the complete set of protein-protein interactions involving the various subunits. The results will be disseminated via peer-reviewed publication as well as through web pages managed by the principal investigators (http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). In addition, mutant Arabidopsis stocks will be deposited with the appropriate stock center(s). The research will contribute to a goal of the 2010 Project ("to determine the function of all genes of a reference species within their cellular, organismal and evolutionary contexts by the year 2010") by cataloguing mutant phenotypes, by defining the network of interactions amongst Arabidopsis polyadenylation factor subunits, and by identifying proteins of unknown function that interact with the polyadenylation machinery.The research will greatly increase the current understanding of mRNA 3' end formation in higher plants. As such, it will enhance the ability to tailor foreign genes for expression in transgenic plants, and thereby develop improved crops (as well as novel alternative economic opportunities) for farmers. It will also lend significant insight into the evolution of mRNA polyadenylation. The research products (cloned cDNA, antibodies, plant lines etc.) will be made available to the research community at large. The research will contribute to the training of two postdoctoral scholars, four graduate students, and as many as twelve undergraduates. The latter will be drawn from highly-regarded and diverse programs at the University of Kentucky and Miami University.