RIG: Characterization of acd6-1 SUPPRESSOR 3 (SUP3), a Negative Regulator of Arabidopsis Defense

RIG：拟南芥防御负调节因子 acd6-1 SUPPRESSOR 3 (SUP3) 的表征

• 批准号: 0818651
• 负责人: Hua Lu
• 金额: $ 17.5万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0818651&HistoricalAwards=false
• 关键词: RIG; Characterization; acd6; SUPPRESSOR; SUP3

Plant diseases caused by pathogens are among the greatest deterrents in agriculture worldwide. A network of plant genes perceives and transduces defense signals in response to pathogen infection. However, it remains challenging to identify genes regulating defense and to understand their mechanisms of action. The Arabidopsis mutant acd6-1 is a tiny plant whose size is in reverse proportion to the level of defense. Taking advantage of this unique feature of acd6-1, the investigators developed a facile genetic screen to isolate acd6-1 suppressors (sups) that may have mutations in novel defense genes. Among the genes identified is SUP3, encoding an uncharacterized putative sodium-dependent phosphate transporter widely conserved in plants and animals. The project will focus on investigating the function of SUP3, using genetic and molecular biology-related tools. The expected results will elucidate how SUP3 regulates plant disease resistance and mediates defense signal transduction. Such information will add to the knowledge on the regulatory network of plant defense and provide a potential target to improve disease resistance traits in crop plants. The project will also contribute to broaden the participation of under-represented groups in Biological Sciences. Minority undergraduate and graduate students will be trained in Plant Biology by being directly involved in the proposed research activities. In addition, K-12 science teachers from low performing public schools in the Baltimore area that have high enrollments of minority students will be invited to work in the principal investigator's laboratory in the summer. Such experience will not only enhance the teachers' knowledge of Biological Sciences but also help them to develop more effective science curricula for their students.

由病原体引起的植物病害是全世界农业中最大的阻碍因素之一。植物基因网络感知并传递防御信号以响应病原体感染。然而，识别调控防御的基因并了解其作用机制仍然具有挑战性。拟南芥突变体acd 6 -1是一个微小的植物，其大小与防御水平成反比。利用acd 6 -1的这一独特特征，研究人员开发了一种简单的遗传筛选方法，分离出可能在新防御基因中发生突变的acd 6 -1抑制基因。在已鉴定的基因中，SUP 3编码一种在植物和动物中广泛保守的未表征的假定钠依赖性磷酸盐转运蛋白。该项目将重点研究SUP 3的功能，使用遗传和分子生物学相关工具。预期的结果将阐明SUP 3如何调节植物抗病性和介导防御信号转导。这些信息将增加对植物防御调控网络的了解，并为改善作物抗病性状提供潜在的目标。该项目还将有助于扩大生物科学中代表性不足的群体的参与。少数民族本科生和研究生将通过直接参与拟议的研究活动进行植物生物学培训。此外，来自巴尔的摩地区少数民族学生入学率高的低绩效公立学校的K-12科学教师将被邀请在夏季在首席研究员的实验室工作。这些经验不但可加强教师对生物科学的认识，亦有助他们为学生发展更有效的科学课程。