The Genetics of Female Acoustic Preference Behavior

克里·L·肖，IOS-0818862：女性发声偏好行为的遗传学。新物种可能是通过交配前信号的变化而形成的。在几个广为人知的物种集群中，不同的交配信号似乎是密切相关的物种保持不同的主要原因。人们对这种进化变化知之甚少。一种假设是，男性交配信号的基因与女性反应(或偏好)的基因相同。这项研究的重点是劳帕拉，这是一组关系密切的板球物种，已经非常迅速地分化。像大多数蟋蟀一样，劳帕拉通过声音交流，声音差异导致不同物种之间的交配不相容。然而，一些物种可以在实验室中杂交，从而能够对这些差异进行遗传研究。两个在雄性鸣叫和雌性偏好上明显不同的密切相关物种将被杂交。杂交后代将与这些物种中的一个交配几代，以将偏好和歌唱基因从一个物种转移到另一个物种。分子标记将被用来制作一张基因组图，显示歌曲和偏好背后的基因位置。有了这个图谱，就有可能确定歌曲和偏好基因是否映射到相同的位置，以及每个基因在多大程度上导致物种之间的差异。这张地图上的分子标记的数量将随后增加，以促进最终克隆与歌曲和偏好相关的基因。通过培养本科生、研究生和博士后学生，研究和教育是相结合的。Kamehameha School的学生将参与建立一个基于网络的科学实验室，分析歌曲的遗传学和偏好遗传。P.I.将访问学校，通过讲座和与学生的非正式互动提供背景信息。

Kerry L. Shaw, IOS-0818862: The genetics of female acoustic preference behavior. New species may be formed by changes in the signals that precede mating. In several well known clusters of species, divergent mating signals appear to be a primary reason why closely related species remain distinct. This sort of evolutionary change is poorly understood. One hypothesis is that the genes for male mating signals are the same as those for female response (or preference). This study focuses on Laupala, a group of closely related cricket species that has diverged very rapidly. Like most crickets, Laupala communicate acoustically, and acoustic differences contribute to mating incompatibility between species. However, some species can be hybridized in the laboratory, enabling a genetic study of these differences. Two closely related species that differ markedly in male song and female preference will be hybridized. Hybrid offspring will be mated with one of these species for several generations to move preference and song genes from one species into the other. Molecular markers will be used to produce a genome map of the location of genes underlying song and preference. With this map, it will be possible to ascertain whether song and preference genes map to the same location and how much each gene contributes to differences between species. The number of molecular markers on this map will subsequently be increased to facilitate the eventual cloning of genes underlying song and preference.Research and education are integrated by training undergraduate, graduate and postdoctoral students. Kamehameha Schools students will be involved by establishing a web-based science lab analyzing the genetics of song and preference inheritance. The P. I. will visit the school to provide context through lecture and informal interaction with students.