Arabidopsis 2010 Project Collaborative Research: Modeling Biological Networks in Arabidopsis through Integration of Genomic, Proteomic, and Metabolomic Data

苯丙素是一类重要的植物次生代谢物，对植物的生长和生存至关重要，其功能包括提供花朵颜色，保护植物免受紫外线照射，防御病原体，以及调节激素运输。这些代谢物也具有重要的医学意义，因为它们在人类中具有抗氧化和抗癌的特性。对苯丙素合成途径的广泛分子和生化表征，结合对代谢物本身生理功能的最新见解，使其成为解决细胞代谢基本问题的理想系统，使用综合的多学科方法将这些分子和生化事件置于生理环境中。了解这一途径的控制将为设计苯丙素和其他植物代谢物的合成提供见解，既可以改善植物生长，又可以促进这些重要化合物的生产，从而提高作物的营养和膳食水平。在这个拟南芥2010项目中，6个研究小组将聚集在一起进行一项独特的发现工作，包括建立生物框架模型，将基因表达、蛋白质表达和代谢物产生整合到拟南芥苯丙氨酸功能、信号和代谢的综合图谱中。本项目通过收集第一批统一的时间过程基因表达、蛋白质表达和代谢物数据集，然后使用新兴的计算方法将这些综合数据集结合起来，以创建集成生物网络的可测试模型，从而解决了2010年项目的优先事项。该项目结合了建模、理论和实验，以产生一个系统水平的理解激素控制转录和调节机制控制苯丙醇生物合成。该项目的研究成果将通过出版物和建立一个信息丰富的网站（http:// www.phenylpropanoid2010.wfu.edu）向公众广泛提供。本项目将培养本科生、研究生和博士后研究人员。此外，ppi将与地方和国家团体合作，加强科学教育，包括发展K-12学生和教师的经验，并为科学领域代表性不足的群体的个人提供研究机会。

Phenylpropanoids are an important class of plant secondary metabolites that are critical for growth and survival, with functions ranging from providing flower color, protection from ultraviolet light, and defense against pathogens, to the regulation of hormone transport. These metabolites are also of medical importance due to their documented anti-oxidant and anti-cancer properties in humans. Extensive molecular and biochemical characterization of the pathway responsible for the synthesis of phenylpropanoids, combined with recent insights into the physiological functions of the metabolites themselves, make this an ideal system to address fundamental questions about cellular metabolism using an integrative, multidisciplinary approach to place these molecular and biochemical events into a physiological context. Understanding the controls of this pathway will provide insights into engineering the synthesis of phenylpropanoids and other plant metabolites, both for improving plant growth and facilitating the production of these important compounds for the nutritional and dietary enhancement of crops. In this Arabidopsis 2010 project, six research groups will come together in a unique discovery effort, involving the establishment of biological framework models that integrate gene expression, protein expression, and metabolite production into a comprehensive map of phenylpropanoid function, signaling, and metabolism in Arabidopsis thaliana. This project addresses the priorities of the 2010 project by collecting one of the first unified sets of time course gene expression, protein expression, and metabolite data and then use emerging computational methods to combine these comprehensive data sets in order to create testable models of integrated biological networks. This project combines modeling, theory, and experimentation to produce a systems-level understanding of the hormonal controls of the transcriptional and regulatory mechanisms controlling phenylpropanoid biosynthesis. Research results from this project will be made widely accessible to the public through publication and through construction of an information-rich website at http:// www.phenylpropanoid2010.wfu.edu. This project will result in interdisciplinary training of undergraduate and graduate students and postdoctoral researchers. In addition, the PIs will partner with local and national groups to enhance science education, including development of K-12 student and teacher experiences and providing research opportunities for individuals from groups underrepresented in science.