2010 Project: Arabidopsis 2010: Transcriptomes for Functional and Evolutionary Studies

2010 项目：拟南芥 2010：用于功能和进化研究的转录组

• 批准号: 0929262
• 负责人: Richard Clark
• 金额: $ 73.78万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0929262&HistoricalAwards=false
• 关键词: 2010; Project; Arabidopsis; Transcriptomes; Functional

A basic challenge in the life sciences is to understand how genetic variation among individuals (DNA sequence differences) leads to variation in traits, for example, eye color in animals or crop yield in plants. Ultimately, trait differences among individuals result from differences in the kind or amount of molecules (for example, RNA and protein molecules) produced by cells. In this project, the investigators will generate a resource to address the fundamental question of how differences in DNA sequences among individuals lead to the production of different RNA molecules, which in turn affect protein types, levels, and whole-organism traits. To do this, the investigators will exploit newly developed technologies, including so-called 'second generation' DNA sequencing methods, to characterize in a highly cost effective manner the majority of RNA molecules from 19 diverse strains of the model plant, Arabidopsis thaliana, for which sequenced genomes are available. A general outcome will be an understanding of how genetic variation affects RNA levels, and hence how DNA differences lead to trait differences. Specifically, the resource will allow investigators from around the United States and the world to understand the regulation of genes relevant to a multitude of traits of critical importance for agriculture in the coming century. The resource of sequence data that will be generated by the project will be released publically via the deposition in the DNA sequence database of the USA National Center for Biotechnology. Internet links to these data, and a description of the project, will be available on website of the lead project investigator within the public University of Utah website. Hands-on training will be provided to each of the undergraduate students, graduate students, and postdoctoral researchers involved in the project. The project will expose trainees to advanced biological technologies and analysis methods of ever increasing importance in biology.

生命科学的一个基本挑战是了解个体之间的遗传变异（DNA序列差异）如何导致性状的变异，例如动物的眼睛颜色或植物的作物产量。最终，个体之间的性状差异是由细胞产生的分子（例如RNA和蛋白质分子）的种类或数量的差异造成的。在这个项目中，研究人员将产生一个资源，以解决个体之间DNA序列的差异如何导致不同RNA分子的产生，从而影响蛋白质类型，水平和整个生物体特征的基本问题。为了做到这一点，研究人员将利用新开发的技术，包括所谓的“第二代”DNA测序方法，以高度成本效益的方式表征来自19种不同株系的模式植物拟南芥的大部分RNA分子，其中测序的基因组是可用的。一般的结果将是理解遗传变异如何影响RNA水平，从而DNA差异如何导致性状差异。具体来说，该资源将使来自美国和世界各地的研究人员能够了解与未来世纪对农业至关重要的众多性状相关的基因调控。该项目将产生的序列数据资源将通过美国国家生物技术中心的DNA序列数据库中的沉积而被释放。这些数据的互联网链接和项目描述将在犹他州公共大学网站内的首席项目研究者网站上提供。将为参与该项目的每个本科生、研究生和博士后研究人员提供实践培训。该项目将使受训人员接触先进的生物技术和生物学中日益重要的分析方法。