MRI: Acquisition of a Laser Scanning Confocal Microscope to Advance Research and Research Training Opportunities at San Francisco State University

MRI：购买激光扫描共焦显微镜以推进旧金山州立大学的研究和研究培训机会

• 批准号: 0821204
• 负责人: Diana Chu
• 金额: $ 63.37万
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821204&HistoricalAwards=false
• 关键词: MRI; Acquisition; Laser; Scanning; Confocal

A grant has been awarded to San Francisco State University (SFSU) under the supervision of Dr. Diana Chu to purchase a Laser Scanning Confocal Microscope for the Cell and Molecular Imaging Center (CMIC). The instrument includes efficient and sensitive spectral detectors to allow analysis of high-resolution, 3-dimensional reconstructions of fluorescently-labeled samples. This will advance studies that uncover mechanisms of embryonic development using Xenopus and chick, intracellular signaling in plants, and the design of cancer therapy compounds. Multiple lasers and precise control over excitation and emission wavelengths will enable the simultaneous use of a broad range of fluorophores that are required to understand chromatin function in worms, gene expression in ants and crabs, and chromosome positioning in mammals. On-site access will allow live imaging to capture rapid chromosome movements in C. elegans and transient cell signals in chick muscle development. The sensitivity, resolution, and speed of this instrument will permit SFSU researchers to employ advanced imaging techniques to address new research questions at the forefront of their respective fields in developmental biology, chemistry and mathematics. The research SFSU scholar-teachers conduct will be integrated with closely mentored training using this system to enhance the career development of SFSU students, 35% of whom are under-represented minorities and 58% women. This diversity is reflected in the 11 investigators included in this proposal, which includes 5 women, 3 Latinos, and 1 Native American. Individual research projects and courses in fluorescence and confocal microscopy will prepare students for success at PhD institutions and biotechnology companies. Affordable access to this system will also enhance collaborations between researchers at neighboring institutions and the diverse faculty and students at SFSU.

美国旧金山州立大学（SFSU）在Diana Chu博士的指导下获得了一笔资金，用于为细胞和分子成像中心（CMIC）购买一台激光扫描共聚焦显微镜。该仪器包括高效、灵敏的光谱探测器，可对荧光标记样品进行高分辨率、三维重建分析。这将有助于揭示爪蟾和鸡的胚胎发育机制、植物细胞内信号传导以及癌症治疗化合物的设计。多个激光器和对激发和发射波长的精确控制将能够同时使用广泛的荧光团，这些荧光团需要了解蠕虫的染色质功能，蚂蚁和螃蟹的基因表达以及哺乳动物的染色体定位。现场访问将允许实时成像捕捉秀丽隐杆线虫的快速染色体运动和鸡肌肉发育中的瞬时细胞信号。该仪器的灵敏度、分辨率和速度将使SFSU的研究人员能够采用先进的成像技术来解决各自领域的前沿研究问题，包括发育生物学、化学和数学。旧金山州立大学学者教师的研究将与密切指导的培训结合起来，利用这个系统来促进旧金山州立大学学生的职业发展，其中35%是少数民族，58%是女性。这种多样性反映在该提案中包括的11名调查人员中，其中包括5名女性，3名拉丁裔和1名印第安人。荧光和共聚焦显微镜的个人研究项目和课程将为学生在博士机构和生物技术公司的成功做好准备。负担得起的系统访问也将加强邻近机构的研究人员与旧金山州立大学不同教职员工和学生之间的合作。