U.S.-Australia Dissertation Enhancement: Cytonuclear Interactions in a Hybrid Parthenogenetic Gecko (Heteronotia binoei)

美国-澳大利亚论文增强：杂交孤雌壁虎（Heteronotia binoei）的细胞核相互作用

• 批准号: 0824991
• 负责人: Craig Moritz
• 金额: $ 1.12万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0824991&HistoricalAwards=false
• 关键词: U.S.; Australia; Dissertation; Enhancement; Cytonuclear

0824991MoritzTitle: U.S.-Australia Dissertation Enhancement: Cytonuclear Interactions in a Hybrid Parthenogenetic Gecko (Heteronotia binoei)This award supports a doctoral dissertation enhancement project between Dr. Craig Moritz and his student Matthew Fujita in the Museum of Vertebrate Zoology at the University of California, Berkeley and Dr. Michael Kearney in the Zoology Department at the University of Melbourne in Australia. Parthenogenetic organisms, which reproduce clonally without sex, are often hybrids with mismatched mitochondrial and nuclear genomes. This perpetual cytonuclear mismatch can have important phenotypic consequences which may alter our view of the paradox of sex. Compared to sexual forms, parthenogenetic geckos of the Heteronotia binoei complex exhibit altered physiological performance and developmental stability, phenotypic traits potentially associated with cytonuclear interactions in the electron transport chain of aerobic respiration. The researchers will investigate disrupted epistatic interactions in the coevolved cytonuclear complexes in parthenogenetic Heteronotia that may have resulted from their hybrid origin and/or subsequent genetic changes. Variation in cytochrome c oxidase activity among four clonal lineages of Heteronotia will be used to examine the interactions of two distinct mitochondrial lineages in different nuclear backgrounds. The data will contribute to an international collaboration integrating genomics, biochemical assays, and physiological data to understand the origin, evolution and phenotypic consequences of hybrid parthenogenesis in Heteronotia binoei, with broader implications on identifying genomic processes in allopolyploid organisms and understanding and resolving the paradox of sex. There is sufficient overlap of interests between the University of California and the University of Melbourne to indicate that they can successfully pursue the activities proposed and that the interaction will benefit both sides. Results from this research are relevant to genome evolution, diversification, speciation (genomic incompatibilities), phenotypic variation (physiology), the evolution and maintenance of sex and the identification of genomic processes in allopolyploid organisms. This research will provide the US graduate student an excellent opportunity to receive a global research experience. It is anticipated that he will maintain contacts and collaborations with the Australian researchers and students throughout his career

0824991 Moritz职务：美国-澳大利亚论文增强：该奖项支持加州大学伯克利分校脊椎动物学博物馆的克雷格莫里茨博士和他的学生Matthew Fujita以及澳大利亚墨尔本大学动物学系的Michael Kearney博士之间的博士论文改进项目。 单性生殖生物，无性繁殖，往往是杂交与不匹配的线粒体和核基因组。 这种永久的细胞核错配可能会产生重要的表型后果，这可能会改变我们对性别悖论的看法。 性形式相比，单性生殖壁虎的Heteronotia binoei复杂的表现出改变的生理性能和发育稳定性，表型性状可能与细胞核的相互作用在电子传递链的有氧呼吸。 研究人员将调查在孤雌生殖Heteronotia中共同进化的细胞核复合体中破坏的上位相互作用，这可能是由于它们的杂交起源和/或随后的遗传变化造成的。 细胞色素c氧化酶活性的变化之间的四个克隆谱系的Heteronotia将被用来检查两个不同的线粒体谱系在不同的核背景的相互作用。 这些数据将有助于整合基因组学，生化测定和生理数据的国际合作，以了解异源多倍体生物中杂交孤雌生殖的起源，进化和表型后果，对识别异源多倍体生物的基因组过程以及理解和解决性别悖论具有更广泛的影响。加州大学和墨尔本大学之间有着充分的利益重叠，表明它们能够成功地开展拟议的活动，这种互动将使双方受益。 这项研究的结果与基因组进化、多样化、物种形成（基因组不相容性）、表型变异（生理学）、性别的进化和维持以及异源多倍体生物体中基因组过程的鉴定有关。 这项研究将为美国研究生提供一个获得全球研究经验的绝佳机会。 预计他将在整个职业生涯中与澳大利亚研究人员和学生保持联系和合作