Enhanced Phytoremediation using Endophytes

CBET-0829027 Doty，Sharon L.华盛顿大学拟议的研究考虑使用快速生长的棉白杨和杨柳对三氯乙烯进行植物修复;生活在这些树木中的内生菌（微生物）将用于加强修复过程。由于PI实验室中分离的许多白杨和杨柳内生菌都可以诱导快速根系生长并固定大气氮，因此这些内生菌的存在可能对这些树种的生存和成功建立起至关重要的作用。该项目是第一次全面研究内生菌对TCE代谢的有益作用，最适合植物修复的两个树属。开发不涉及基因工程的提高树木修复污染场地的能力的方法将有助于使这种更环保的技术更广泛地被接受。PI和她的十个实验室成员中的七个是女性科学家。本科研究人员将通过华盛顿大学少数民族计划招募。作为国际白杨委员会的积极成员和白杨和杨柳的环境用途工作组的一部分，PI与世界各地的白杨和杨柳用户有直接联系，以确保该项目产生的知识将被迅速利用，以环境可持续的方式改善污染物的补救。

CBET- 0829027Doty, Sharon L.University of WashingtonThe proposed research considers phytoremediation of TCE using fast-growing cottonwood and willow trees; endophytes (microorganisms) living within these trees will be used to enhance the remediation processes. Since many of the poplar and willow endophytes isolated in the PI's laboratory can both induce rapid root growth and fix atmospheric nitrogen, the presence of these endophytes may have a vital role in the survival and successful establishment of these tree species. This project is the first comprehensive study of the beneficial role of endophytes on TCE metabolism by two tree genera most suitable for phytoremediation. Development of methods of improving the ability of trees to remediate polluted sites that do not involve genetic engineering will aid in making this greener technology more accepted for widespread use. The PI and seven of her ten lab members are female scientists. Undergraduate researchers will be recruited through the UW Minority Program. As an active member of the International Poplar Commission and part of the Working Group on environmental uses of poplar and willow, the PI has direct connections to users of poplar and willow around the world to ensure that the knowledge generated in this project will be utilized promptly to improve remediation of pollutants in an environmentally sustainable way.