Establishment of phytoremediation technologies of contaminated soils using heavy metal accumulating woody plants

柳树（Salix sp.）植物具有吸收大量有毒金属的能力。在这项工作中，我研究了柳树植物对环境中金属污染的修复潜力。利用同步辐射x射线微量分析等理化证据，确定了植物体内金属的积累位点和形态。构建组织特异性cDNA文库，鉴定金属积累和解毒基因。还研究了生长在老矿区附近的柳树，以评价该植物在田间的金属积累能力。

Willow(Salix sp.) plants have the capability to take up high levels of toxic metals. In this work, I have investigated the phytoremediation potential of the willow plants to clean up the metal pollution in the environment. Several accumulation sites in the plant body and speciation of the endogenous metals were identified by using physicochemical evidences including synchrotron-radiation based X-ray microanalyses. Tissue specific cDNA libraries were constructed to identify the genes for metal accumulation and detoxification. Willow plants growing near an old mining site was also examined to evaluate the metal accumulating ability of this plant in the field.