Permanent sterile Oberflächen durch Kombination von antimikrobiellen und Biobelag verhindernden Oberflächenstrukturen

通过抗菌和防生物污垢表面结构的结合实现永久无菌表面

• 批准号: 189968166
• 负责人: Professorin Dr. Karen Lienkamp
• 金额: --
• 依托单位: Professur für Polymerwerkstoffe
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/189968166?language=en
• 关键词: Permanent; sterile; Oberfl; chen; durch

To overcome the deficiencies of today's antibacterial materials, the aim of this project is to develop long-term sterile polymer surfaces that are simultaneously antibacterial and antibiofouling. While the antibacterial component will kill pathogens, the antibiofouling component will make the surface self-cleaning by preventing irreversible attachment of proteins, cells or cell debris, and thus the formation of a biofilm. To make such surfaces, we need a thorough understanding of how antimicrobial polymer surfaces kill bacterial cells, and how antibiofouling polymers prevent adhesion. Thus, we need to know how each surface parameter (chemical composition, cation type and charge density, hydrophobicity, patch size, layer thickness, grafting density, roughness, wettability, swellability etc.) and each environmental parameter (pH, ionic strength, buffer composition, temperature etc.) influences the surface-cell interaction. Therefore, besides the long-term aim to obtain the perfectly sterile surface, the aim of this project is to increase the fundamental understanding of polymer surface-cell interactions. We follow these approaches: (1) We will identify antimicrobial polyelectrolytes that do not form irreversible complexes with phospholipids, lipopolysaccharide, peptidoglycan, and other cell debris, and use them as the antibacterial component, and (2) We will synthesize nanostructured surfaces from mixed antimicrobial-antibiofouling polymers. Time permitting, we will integrate antimicrobial polymers into layered, enzymatically degradable, self-regenerating surfaces.

为了克服当今抗菌材料的不足，该项目的目标是开发同时具有抗菌和抗生物污垢的长期无菌聚合物表面。抗菌成分将杀死病原体，而抗生物污垢成分将通过防止蛋白质、细胞或细胞碎片的不可逆附着而使表面自我清洁，从而形成生物膜。为了制造这样的表面，我们需要彻底了解抗菌聚合物表面是如何杀死细菌细胞的，以及抗生物污垢聚合物是如何防止粘连的。因此，我们需要知道每个表面参数(化学成分、阳离子类型和电荷密度、疏水性、贴片大小、层厚度、接枝密度、粗糙度、润湿性、膨胀性等)是如何变化的。以及各环境参数(pH、离子强度、缓冲液成分、温度等)。影响表面与细胞的相互作用。因此，除了获得完全无菌表面的长期目标外，该项目的目的是增加对聚合物表面-细胞相互作用的基本了解。我们将采用以下方法：(1)我们将识别与磷脂、内毒素、肽多聚糖和其他细胞碎片不形成不可逆络合物的抗菌聚电解质，并将其用作抗菌组分；(2)我们将从抗菌-抗生物污垢混合聚合物中合成纳米结构表面。如果时间允许，我们将把抗菌聚合物集成到分层的、可酶降解的、自我再生的表面上。

项目成果

Polymer-Based Surfaces Designed to Reduce Biofilm Formation: From Antimicrobial Polymers to Strategies for Long-Term Applications.

• DOI: 10.1002/marc.201700216
• 发表时间: 2017-10
• 期刊: Macromolecular rapid communications
• 影响因子: 4.6
• 作者: Riga EK;Vöhringer M;Widyaya VT;Lienkamp K
• 通讯作者: Lienkamp K

Fluorescent ROMP Monomers and Copolymers for Biomedical Applications.

• DOI: 10.1002/macp.201700273
• 发表时间: 2017-11
• 期刊: Macromolecular chemistry and physics
• 影响因子: 2.5
• 作者: Esther K. Riga;David Boschert;M. Vöhringer;Vania Tanda Widyaya;Monika Kurowska;W. Hartleb;Karen Lienkamp

Just Antimicrobial is not Enough: Toward Bifunctional Polymer Surfaces with Dual Antimicrobial and Protein-Repellent Functionality

• DOI: 10.1002/macp.201500266
• 发表时间: 2016-01-01
• 期刊: MACROMOLECULAR CHEMISTRY AND PHYSICS
• 影响因子: 2.5
• 作者: Hartleb, Wibke;Saar, Julia S.;Lienkamp, Karen
• 通讯作者: Lienkamp, Karen