SGER: Adenovirus UV Treatment - Exploration of fundamental mechanisms of disinfection

CBET-0832338K。林登控制饮用水中的病毒对公众健康至关重要，而消毒是抵御致病微生物的主要屏障。紫外线消毒现在是废水的首选方法，由于对氯化副产物的关注和对隐孢子虫灭活的需要，紫外线消毒正在成为大小饮用水系统消毒的非常重要的工具。使用过去10年使用254 nm低压紫外线消毒对腺病毒进行紫外线治疗的细胞培养感染性数据，将2006年美国环保局对所有病毒的消毒要求设定在几乎是实际使用的典型紫外线剂量40MJ/cm2的5倍的水平。然而，PI和各种合作研究人员最近的研究发现，使用较新的多色UV源(中压和脉冲UV)显著提高了腺病毒的UV消毒效果。紫外线光源之间的这些差异表明，对紫外线照射如何影响腺病毒缺乏基本的了解。许多作者呼吁加强对病毒对紫外线反应的基本分子机制的了解，以及准确检测病原体的分子方法。这项研究将加深对紫外线消毒病毒背后机理的理解，并将为进一步的消毒调查提供强有力的工具，取得保护公众健康的重要积极结果。

CBET-0832338K. LindenControl of viruses in drinking water is critical for public health, and disinfection is the primary barrier against disease-causing microorganisms. UV disinfection is now the method of choice for wastewater and is becoming a very important tool for disinfection of large and small drinking water systems due to the concerns over chlorination byproducts and the need to inactivate Cryptosporidium. Cell culture infectivity data generated over the past 10 years on UV treatment of adenoviruses using 254 nm low-pressure (LP) UV disinfection was used to set 2006 US EPA standards for disinfection requirements of all viruses at a level almost 5 times the typical UV dose of 40 mJ/cm2 used in practice. However, recent research by the PI and various co-investigators has found that use of newer polychromatic UV sources (medium pressure [MP] and pulsed UV) significantly improves the UV disinfection of adenoviruses. These differences between UV sources indicate that a fundamental understanding of how UV irradiation affects adenoviruses is lacking. Numerous authors have called for an increased understanding of the fundamental molecular mechanisms involved in viral response to UV as well as molecular methods for accurate pathogen detection. This research will enhance the understanding of the mechanisms behind UV disinfection of viruses and methods used will provide powerful tools for further disinfection investigations with important positive results for protection of public health.