SGER: Adenovirus UV Treatment - Exploration of fundamental mechanisms of disinfection

SGER：腺病毒紫外线处理——消毒基本机制的探索

• 批准号: 0832338
• 负责人: Karl Linden
• 金额: --
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0832338&HistoricalAwards=false
• 关键词: SGER; Adenovirus; UV; Treatment; Exploration

CBET-0832338K. LindenControl of viruses in drinking water is critical for public health, and disinfection is the primary barrier against disease-causing microorganisms. UV disinfection is now the method of choice for wastewater and is becoming a very important tool for disinfection of large and small drinking water systems due to the concerns over chlorination byproducts and the need to inactivate Cryptosporidium. Cell culture infectivity data generated over the past 10 years on UV treatment of adenoviruses using 254 nm low-pressure (LP) UV disinfection was used to set 2006 US EPA standards for disinfection requirements of all viruses at a level almost 5 times the typical UV dose of 40 mJ/cm2 used in practice. However, recent research by the PI and various co-investigators has found that use of newer polychromatic UV sources (medium pressure [MP] and pulsed UV) significantly improves the UV disinfection of adenoviruses. These differences between UV sources indicate that a fundamental understanding of how UV irradiation affects adenoviruses is lacking. Numerous authors have called for an increased understanding of the fundamental molecular mechanisms involved in viral response to UV as well as molecular methods for accurate pathogen detection. This research will enhance the understanding of the mechanisms behind UV disinfection of viruses and methods used will provide powerful tools for further disinfection investigations with important positive results for protection of public health.

CBET-0832338K。饮用水中病毒的Lindencontrol对于公共卫生至关重要，消毒是防止引起疾病的微生物的主要障碍。紫外线消毒现在是废水的首选方法，由于对氯化副产物的担忧以及需要灭活隐孢子虫的关注，因此正在成为对大小饮用水系统消毒的非常重要的工具。在过去的10年中，使用254 nm低压（LP）紫外线消毒在过去10年中生成的细胞培养感染数据用于设定2006年美国EPA标准所有病毒的消毒需求，几乎是使用40 MJ/CM2使用的典型uv剂量的近5倍。然而，PI和各种共同投资者的最新研究发现，使用新的多色紫外线源（中压[MP]和脉冲紫外线）可显着改善腺病毒的紫外线消毒。紫外线来源之间的这些差异表明，对紫外线辐射如何影响腺病毒的基本了解。许多作者呼吁人们对参与病毒反应涉及的紫外线反应的基本分子机制以及用于准确病原体检测的分子方法有更多的了解。这项研究将增强对紫外线消毒病毒和所使用方法背后的机制的理解，将为进一步的消毒调查提供强大的工具，并为保护公共卫生而重要的积极成果。