Intrinsic Tilt of Transmembrane Helices

跨膜螺旋的固有倾斜

• 批准号: 0841227
• 负责人: Roger Koeppe
• 金额: $ 58.03万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0841227&HistoricalAwards=false
• 关键词: Intrinsic; Tilt; Transmembrane; Helices

The experiments for this project will employ solid-state magnetic resonance methods to investigate peptide-lipid interactions. In particular, the investigators seek to understand the molecular mechanisms by which designed, anchored, membrane-spanning peptides interact with lipids and adjust their tilt or geometry in response to changes in the lipid environment. Of specific importance will be attempts to resolve recent apparent discrepancies or controversies that have arisen between the experimental results from magnetic resonance spectroscopy and the theoretical predictions from molecular dynamics simulations concerning the behavior of peptides that span lipid bilayer membranes. The experimental methods will address these timely issues by using two independent and inherently different approaches, namely methods based upon the deuterium (2H) quadrupole interaction and the nitrogen/hydrogen (15N/1H) dipole interactions. The expected results will enhance the collective understanding of protein/lipid interactions and the function of membrane proteins at the molecular level.The project will augment a particular University of Arkansas initiative aimed toward developing a new paradigm for undergraduate science education. This HHMI funded "Studio Approach to Science Education" has been designed for innovative recruiting of new creative minds to lifelong careers dedicated to scientific discovery, while at the same time broadening the base for participation of non-traditional individuals and underrepresented groups in science. Efforts here will broaden the team research concept by including NSF-supported students working alongside and in conjunction with ongoing studio research team members. The impact of extending the novel studio/team experience will benefit society by helping to build the future scientific work force.

本项目的实验将采用固态磁共振方法来研究肽-脂质相互作用。特别是，研究人员试图了解设计的、锚定的、跨膜肽与脂质相互作用的分子机制，并根据脂质环境的变化调整其倾斜或几何形状。特别重要的将是试图解决最近在磁共振波谱的实验结果和分子动力学模拟关于跨脂质双层膜的肽行为的理论预测之间出现的明显差异或争议。实验方法将采用两种独立且本质上不同的方法来解决这些问题，即基于氘（2H）四极相互作用和氮/氢（15N/1H）偶极相互作用的方法。预期的结果将增强对蛋白质/脂质相互作用和膜蛋白在分子水平上的功能的集体理解。该项目将加强阿肯色大学的一项特别倡议，该倡议旨在为本科科学教育开发一种新范式。这项由HHMI资助的“工作室式科学教育”旨在以创新的方式招募新的有创造力的人，终身致力于科学发现，同时扩大非传统个人和未被充分代表的群体参与科学的基础。这里的努力将扩大团队研究概念，包括nsf支持的学生与正在进行的工作室研究团队成员一起工作。扩展新的工作室/团队经验的影响将通过帮助建立未来的科学工作队伍而造福社会。