Acquisition and Internalization of Copper by Methanotrophs

甲烷氧化菌对铜的获取和内化

基本信息

  • 批准号:
    0842366
  • 负责人:
  • 金额:
    $ 61.7万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-07-15 至 2013-06-30
  • 项目状态:
    已结题

项目摘要

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). Methanobactin (Mb) is a small copper-chelating molecule isolated from methanotrophic bacteria, organisms that utilize methane as their sole carbon source. Methanobactin facilitates copper uptake and may also function in regulation of methane monooxygenase (MMO) enzyme expression, catalysis by the copper-containing, particulate MMO (pMMO), and defense against copper toxicity and resultant oxidative stress. Although some insight into Cu-Mb structure and function has been gained in the past few years, major questions remain unanswered. First, Cu-Mb has only been characterized from one organism. It is not known whether Mb molecules from different species have similar structures. Cu-Mb from three types of methanotrophs will be isolated and characterized by analytical, spectroscopic, and structural methods. These data will provide insight into the structural diversity of Mb and into its role in interspecies competition for metal ions. Second, it is clear that methanotrophs secrete Mb into their growth medium when deprived of copper, but it is not known how Cu-Mb is taken back into the cell. Direct uptake of Cu-Mb will be established experimentally and a variety of biophysical, biochemical, and genetic approaches will be utilized to identify proteins involved in Cu-Mb uptake. Broader ImpactThe growth of methanotrophs, Mb isolation, and spectroscopic characterization have proven to be excellent training projects. Several undergraduate students will work on isolating Mb from different organisms. In addition to exposing undergraduates to this highly collaborative, interdisciplinary research project, underrepresented minority students will have the opportunity to participate as graduate students at Northwestern and through the Chicago State University-NU Masters to the PhD Bridge Program. An understanding of how methanotrophs acquire essential copper ions for methane oxidation will impact greenhouse gas models for various environments, particularly as pertains to acquisition of copper from mineral sources. In addition, Mb is potentially applicable in the semiconductor industry to remove copper from wastewater.
该奖项是根据2009年《美国复苏和再投资法案》(公法111-5)提供资金的。甲烷结核蛋白(Mb)是从甲烷营养细菌中分离出来的一种小的铜螯合分子,这些细菌利用甲烷作为唯一的碳源。甲烷结合蛋白促进铜的摄取,也可能在调节甲烷单加氧酶(MMO)的表达,被含铜的颗粒MMO(PMMO)催化,以及防御铜毒性和由此产生的氧化应激中发挥作用。尽管在过去的几年里已经对铜-Mb的结构和功能有了一些深入的了解,但主要的问题仍然没有得到解答。首先,只有一种生物具有铜-Mb的特征。目前尚不清楚不同物种的Mb分子是否具有相似的结构。将从三种类型的甲烷氧化菌中分离出铜-Mb,并用分析、光谱和结构方法进行表征。这些数据将为深入了解Mb的结构多样性以及它在物种间对金属离子的竞争中所起的作用提供依据。其次,很明显,甲烷氧化菌在缺乏铜的情况下会将Mb分泌到它们的生长介质中,但尚不清楚铜-Mb是如何被带回细胞的。将在实验中建立对铜-Mb的直接吸收,并将利用各种生物物理、生化和遗传方法来识别参与铜-Mb吸收的蛋白质。更广泛的影响甲烷氧化菌的生长、Mb的分离和光谱表征已被证明是很好的培训项目。几名本科生将致力于从不同的生物体中分离出Mb。除了让本科生参与这个高度协作的跨学科研究项目外,未被充分代表的少数族裔学生将有机会作为研究生参加西北大学,并通过芝加哥州立大学-NU硕士到博士桥梁项目。了解甲烷氧化菌如何获取甲烷氧化所必需的铜离子将影响各种环境下的温室气体模型,特别是与从矿物来源获取铜有关的模型。此外,Mb在半导体工业中用于去除废水中的铜具有潜在的应用价值。

项目成果

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Amy Rosenzweig其他文献

Amy Rosenzweig的其他文献

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{{ truncateString('Amy Rosenzweig', 18)}}的其他基金

NSF-BSF: Novel determinants of prokaryotic copper homeostasis
NSF-BSF:原核铜稳态的新决定因素
  • 批准号:
    1938715
  • 财政年份:
    2020
  • 资助金额:
    $ 61.7万
  • 项目类别:
    Standard Grant
Acquisition of Instrumentation for Macromolecular Crystallography at the Dupont-Northwestern-Dow Collaborative Access Team Beamlines
杜邦-西北-陶氏合作访问团队光束线购置大分子晶体学仪器
  • 批准号:
    0079340
  • 财政年份:
    2000
  • 资助金额:
    $ 61.7万
  • 项目类别:
    Standard Grant
Structural Biology of Bacterial Copper Resistance
细菌铜抗性的结构生物学
  • 批准号:
    9874408
  • 财政年份:
    1999
  • 资助金额:
    $ 61.7万
  • 项目类别:
    Continuing Grant

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