Collaborative Research: Use of genome enabled tools to understand symbiosis

合作研究：使用基因组工具来理解共生

• 批准号: 0843633
• 负责人: Cheryl Whistler
• 金额: $ 77.27万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0843633&HistoricalAwards=false
• 关键词: Collaborative; Research; Use; genome; enabled

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Cheryl A WhistlerCollaborative Research: Use of genome enabled tools to understand symbiosis IOS- 0843633 The squid-Vibrio fischeri symbiosis has emerged as an engaging and important model for studying animal colonization by bacteria. The availability of several sequenced genomes in the genus Vibrio, has made this a more powerful system than ever for elucidating the complex gene networks that enable the contrasting lifestyles of bacteria. For this molecular genetics project which integrates research and education, the investigators will construct mutant bacteria with defects in each of the more than four thousand bacterial genes present in V. fischeri. This tool for discovery will then be used to characterize bacterial traits relevant to animal colonization in high-throughput assays by six research teams comprised almost entirely of undergraduate researchers. This library of mutants, along with data from the multiple parallel assays, will provide a high-resolution network of functionally-related datasets indexed by gene that will provide an unprecedented view of gene function in the context of the entire genomes. This library of mutants will be publicly available for bioinformatics analysis, and thus will fuel the next generation of experiments. Research increasingly involves high-throughput molecular genetic and genomics-based strategies for discovery that often necessitate the coordination of large teams. Therefore, the large number of students participating in this project will be well prepared for science careers in the 21st century.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。谢丽尔·A·惠斯勒合作研究：利用基因组工具理解共生IOS- 0843633鱿鱼-费氏弧菌共生已经成为研究细菌在动物中定植的一个吸引人的重要模型。弧菌属中几个测序基因组的可用性，使其成为一个比以往任何时候都更强大的系统，用于阐明复杂的基因网络，使细菌的生活方式形成鲜明对比。对于这个集研究和教育于一体的分子遗传学项目，研究人员将构建在V. fischeri中存在的4000多个细菌基因中的每一个都有缺陷的突变细菌。然后，这种发现工具将用于表征与动物定植相关的细菌性状，这些细菌性状由几乎完全由本科研究人员组成的六个研究小组进行高通量测定。该突变体库沿着来自多个平行测定的数据将提供由基因索引的功能相关数据集的高分辨率网络，这将在整个基因组的背景下提供前所未有的基因功能视图。这个突变体库将公开用于生物信息学分析，从而为下一代实验提供动力。研究越来越多地涉及高通量分子遗传学和基于基因组学的发现策略，这往往需要大型团队的协调。因此，参加这个项目的大量学生将为世纪的科学生涯做好准备。