Collaborative Research: Use of genome enabled tools to understand symbiosis

该奖项由2009年美国复苏和再投资法案(公法第111-5条)资助。谢丽尔·惠斯勒合作研究：使用基因组工具了解共生IOS-0843633鱿鱼-鱼弧菌共生已成为研究细菌引起的动物定居的一个吸引人的重要模型。弧菌属几个测序基因组的获得，使这一系统成为比以往任何时候都更强大的系统，用于阐明使细菌的生活方式截然不同的复杂基因网络。对于这个集研究和教育为一体的分子遗传学项目，研究人员将构建突变细菌，该细菌存在于费氏弧菌中的4000多个细菌基因中的每一个都存在缺陷。然后，这一发现工具将被用于在高通量分析中表征与动物定居相关的细菌特征，由几乎全部由本科生研究人员组成的六个研究小组组成。这个突变库，连同来自多个平行分析的数据，将提供一个由基因索引的功能相关数据集的高分辨率网络，将在整个基因组的背景下提供前所未有的基因功能视图。这个突变体库将公开用于生物信息学分析，因此将为下一代实验提供燃料。研究越来越多地涉及高通量的分子遗传学和基于基因组学的发现策略，这往往需要大型团队的协调。因此，参与这一项目的大量学生将为21世纪的科学事业做好准备。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Cheryl A WhistlerCollaborative Research: Use of genome enabled tools to understand symbiosis IOS- 0843633 The squid-Vibrio fischeri symbiosis has emerged as an engaging and important model for studying animal colonization by bacteria. The availability of several sequenced genomes in the genus Vibrio, has made this a more powerful system than ever for elucidating the complex gene networks that enable the contrasting lifestyles of bacteria. For this molecular genetics project which integrates research and education, the investigators will construct mutant bacteria with defects in each of the more than four thousand bacterial genes present in V. fischeri. This tool for discovery will then be used to characterize bacterial traits relevant to animal colonization in high-throughput assays by six research teams comprised almost entirely of undergraduate researchers. This library of mutants, along with data from the multiple parallel assays, will provide a high-resolution network of functionally-related datasets indexed by gene that will provide an unprecedented view of gene function in the context of the entire genomes. This library of mutants will be publicly available for bioinformatics analysis, and thus will fuel the next generation of experiments. Research increasingly involves high-throughput molecular genetic and genomics-based strategies for discovery that often necessitate the coordination of large teams. Therefore, the large number of students participating in this project will be well prepared for science careers in the 21st century.