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Design and Evaluation of DNA-Barcoding High Throughput Methods for Analyzing Diatom Diversity: a Test Case Along a South-North Gradient in Central Europe (Rivers Neiße/Oder)

用于分析硅藻多样性的 DNA 条形码高通量方法的设计和评估：沿中欧南北梯度的测试案例（内河/奥德河）

基本信息

批准号：
191284038
负责人：
Professorin Dr. Birgit Gemeinholzer, since 3/2014
金额：
--
依托单位：
Institut für Biochemie I
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/191284038?language=en
关键词：
Design Evaluation DNA Barcoding Throughput

项目摘要

Water quality and seasonal fiuctuations influence diatom occurrences and abundances. Often organism detection is incomplete or wrong pinpointing to the demand of method amelioration. We aim to establish and evaluate routine DNA-Barcoding high throughput methods for the detection of diatom biodiversity along a south-north gradient in Germany (Neiße/Oder). In 2 Vi years 49 plankton samples will be taken at 7 locations along the river system in 3 different seasons. DNA-Barcodes from mixed water samples of the spring collection, amplified and sequenced via 454 technology and aligned to a reference database, will be compared to results from analyses via light microscopy and SEM from all sites. We will evaluate, whether (1) standardisation of DNA-Barcoding methods for assessing diatom diversity is possible (quantification and/or qualitative detection), (2) new insights into occurrence, distribution and frequency of diatoms along a south-north gradient in the model river system during different seasons can be obtained, (3) the method is applicable for routine water monitoring, (4) the detected genotypic variation can be assigned to voucher samples, and (5) novel diatom species can be described due to genotypic variation despite morphologically homologous traits.

水质和季节波动影响硅藻的发生和丰度。针对微生物检测方法改进的需求，经常出现微生物检测不完整或错误的情况。我们的目标是建立和评估常规DNA条形码高通量方法检测硅藻生物多样性沿着南北梯度在德国（内/奥得河）。在2 Vi年的49浮游生物样品将采取7个地点沿着河流系统在3个不同的季节。来自春季采集的混合水样的DNA条形码，通过454技术进行扩增和测序，并与参考数据库进行比对，将与来自所有地点的光学显微镜和SEM分析结果进行比较。我们将评估（1）评估硅藻多样性的DNA条形码方法的标准化是否可行（定量和/或定性检测），（2）可以获得不同季节模型河流系统中硅藻沿着南北梯度的发生、分布和频率的新见解，（3）该方法适用于常规水质监测，（4）检测到的基因型变异可以被分配给凭证样品，以及（5）尽管形态上同源的性状，但由于基因型变异，可以描述新的硅藻物种。