Stable Isotope Probing to Assess Bioremediation of LUST Contaminants: Addressing Existing MTBE/BTEX and Probable Future Ethanol/BTEX Contamination

通过稳定同位素探测评估 LUST 污染物的生物修复：解决现有的 MTBE/BTEX 和未来可能出现的乙醇/BTEX 污染

• 批准号: 0853249
• 负责人: Alison Cupples
• 金额: $ 30万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0853249&HistoricalAwards=false
• 关键词: Stable; Isotope; Probing; Assess; Bioremediation

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."0853249CupplesLeaking underground storage tanks (LUST) sites have resulted in BTEX (benzene, toluene, ethylbenezene and xylenes) compounds, oxygenates such as methyl tert-butyl ether (MTBE) or tert-butyl alcohol (TBA) migrating into surface and groundwater and eventually show up in drinking water supplies. Even though MTBE is being phased out, the problem is not likely to be resolved soon because of its historical widespread use, the recalcitrant nature of MTBE, and the physiochemical properties of the compound that favor movement to water supplies. Bioremediation through natural attenuation is the most favored cost-effective way of cleanup. However, information on the microorganisms involved at different sites and their ability to efficiently transform these chemicals under various environmental conditions is still lacking.The principal objective of this proposal is to develop and apply stable isotope probing (STP) methods to identify and to study the microorganisms responsible for the transformation of contaminants from leaking underground storage tanks (LUST) under in situ conditions. Major research tasks are: (1) to address the limitations associated with STP and further refining methods accuracy, (2) to apply STP to identify the organisms responsible for the biodegradation of BTEX, MTBE, and TBA, and (3) to determine the impact of in situ conditions on the populations and activity of these microorganisms. The proposed work builds on s strong set of techniques already developed in the PI?s laboratory and is national interest because of the potential contribution to better understanding and the solution of water pollution by leaking LUST. The PI has made a strong attempt to develop the broader impacts of the research and has laid out a detailed set of plans for outreach to underrepresented groups, K-12 education, and international collaborators. Information gained by this project will be broadly disseminated. The proposed international collaboration with a group in Newcastle that is exploring a related topic on biodesel will be very enriching experience for the researchers involved in this effort.

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。“0853249 Cupples泄漏的地下储罐（LUST）场地导致BTEX（苯、甲苯、乙苯和二甲苯）化合物、含氧化合物（如甲基叔丁基醚（MTBE）或叔丁醇（TBA））迁移到地表和地下水中，并最终出现在饮用水供应中。尽管甲基叔丁基醚正在逐步淘汰，但由于其历史上的广泛使用，甲基叔丁基醚的抗柠檬酸性质以及该化合物有利于向供水系统移动的理化性质，该问题不太可能很快得到解决。 通过自然衰减的生物修复是最受欢迎的具有成本效益的清理方式。然而，在不同的网站和他们的能力，以有效地转化这些化学品在各种环境条件下，涉及的微生物的信息仍然是lacked.The主要目标，本提案是开发和应用稳定同位素探测（STP）的方法，以确定和研究的微生物负责的污染物从泄漏的地下储罐（LUST）在原位条件下的转化。主要研究任务是：（1）解决与STP相关的局限性，并进一步改进方法的准确性，（2）应用STP来识别负责BTEX、MTBE和TBA生物降解的生物，以及（3）确定原位条件对这些微生物种群和活性的影响。拟议的工作建立在s强大的一套技术已经开发的PI？这是一个国家利益，因为它可能有助于更好地理解和解决泄漏的LUST造成的水污染。PI已经做出了强有力的尝试，以发展研究的更广泛的影响，并制定了一套详细的计划，以推广到代表性不足的群体，K-12教育和国际合作者。通过这一项目获得的信息将得到广泛传播。拟议中的与纽卡斯尔一个正在探索生物荒漠相关主题的小组的国际合作将为参与这一努力的研究人员提供非常丰富的经验。