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Chitosan Fiberbased Threedimensional Hybrid Scaffolds for Bone Tissue Engineering

用于骨组织工程的基于壳聚糖纤维的三维混合支架

基本信息

批准号：
191760137
负责人：
Professor Dr.-Ing. Chokri Cherif
金额：
--
依托单位：
Max Bergmann Zentrum für Biomaterialien
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/191760137?language=en
关键词：
Chitosan Fiberbased Threedimensional Hybrid Scaffolds

项目摘要

Novel NSN-Hybrid-Scaffolds made of chitosan micro- and nanofibres which are constructed in hierarchically simple scaffold structures with defined porosity, were successfully developed in the first stage of funding. Their suitability as a 3D-scaffold structure for bone TE was tested in vitro. Based on the extensive and promising results of the first stage of funding novel, complexly structured and functionalized NSN-Hybrid-Scaffolds with local adjustable porosities are to be developed in the proposed second stage of funding, in order to generate a scaffold, which has the same structure and material composition as the bone tissue. Therefore, the main objective is the development of textile technological solutions for the realization of such complex collagen-coated NSN-Hybrid-Scaffolds with local adjustable pore gradients and both numerically definable and reproducible properties. For this purpose, the properties of the complex NSN structures shall be modelled by means of computer systems in order to predict the relationships between the applied fibre types, fabrication parameters and coatings and the thus resulting structure and porosity as well as to drive the design guidelines for NSN-Hybrid-Scaffolds. A continuous process chain that uses the algorithms developed in the material model must be established, allowing the defined and pre-set fabrication of complexly structured NSN-Hybridscaffolds and thus minimizing trial & error attempts. A new quantitative method must be developed to analyse the pore size gradients in a statistically valid way. The application of collagen coating for the generation of pore gradients, which is also advantageous for cell response, requires a further study of the influential parameters in the coating of NSN-Hybridscaffolds. A further improvement in cell adhesion, proliferation and differentiation is intended by the targeted mineralization of NSN-Hybrid-Scaffolds and functionalization with growth factors (BMP-2, or VEGF) on the nanofibre-scale. The absorption kinetics of the NSN-Scaffolds will be investigated by varying the fibre types and sterilization methods. By means of fluorescence labelling of the chitosan fibres within the NSN-Hybrid-Scaffolds the bioresorption will be studied in the presence of bone resorbing osteoclasts to obtain conclusions regarding the in vivo degradation behaviour. The most suitable scaffold functionalization for bone cells will be determined by cell culture experiments in mono- and co-cultures. In preparation for in vivo studies the influence of the NSN-Scaffolds on the formation of blood vessels will be examined. As a result of this research work a NSN model scaffold shall be provided, which seems promising for future in vivo experiments.

在第一阶段的资助中，成功开发了由壳聚糖微纤维和纳米纤维制成的新型NSN混合支架，这些纤维以具有定义孔隙率的分层简单支架结构构建。在体外测试了它们作为骨TE的3D支架结构的适用性。基于第一阶段资助的广泛和有希望的结果，在拟议的第二阶段资助中将开发具有局部可调节孔隙率的新型、复杂结构和功能化的NSN-混合-支架，以产生与骨组织具有相同结构和材料组成的支架。因此，主要目标是开发纺织技术解决方案，以实现具有局部可调孔梯度和数值可定义和可再现特性的这种复杂胶原蛋白涂层NSN-混合-支架。为此，应通过计算机系统对复杂NSN结构的特性进行建模，以预测所应用的纤维类型、制造参数和涂层与由此产生的结构和孔隙率之间的关系，并推动NSN混合支架的设计指南。必须建立一个使用材料模型中开发的算法的连续工艺链，允许定义和预设复杂结构的NSN-Hybridscaffolds的制造，从而最大限度地减少试错尝试。必须开发一种新的定量方法，以统计有效的方式分析孔径梯度。胶原涂层的应用产生的孔隙梯度，这也有利于细胞的反应，需要进一步研究的影响参数的涂层的NSN Hybridscaffolds。细胞粘附、增殖和分化的进一步改善旨在通过NSN-杂交-支架的靶向矿化和用生长因子（BMP-2或VEGF）在纳米纤维尺度上的功能化来实现。将通过改变纤维类型和灭菌方法研究NSN支架的吸收动力学。通过荧光标记NSN-杂化-支架内的壳聚糖纤维，将在骨吸收破骨细胞存在下研究生物吸收，以获得关于体内降解行为的结论。用于骨细胞的最合适的支架功能化将通过单培养物和共培养物中的细胞培养实验来确定。在体内研究的准备中，将检查NSN-支架对血管形成的影响。作为这项研究工作的结果，应提供一个NSN模型支架，这似乎是有前途的未来在体内实验。