Regulation of organohalide respiration in Dehalococcoides mccartyi strains

Dehalococcoides mccartyi 菌株中有机卤化物呼吸的调节

• 批准号: 191842164
• 负责人: Privatdozentin Dr. Ute Lechner
• 金额: --
• 依托单位: Institutsbereich Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/191842164?language=en
• 关键词: Regulation; organohalide; respiration; Dehalococcoides; mccartyi

The major aim of this project is to characterize the mechanism of regulation of reductive dehalogenase (rdh) gene expression, which enables Dehalococcoides to respond specifically to diverse chloroorganic compounds and to perform multi-step dechlorination reactions. The project will focus on the biochemical study of putative regulatory proteins encoded in the genome of Dehalococcoides sp. strain CBDB1 by genes divergently transcribed to rdhA genes. The roles of MarR-type regulators and of two-component-system regulators will be addressed. Putative MarR-type regulators will be selected for biochemical analysis on the basis of the observation of a differential transcription of the multiple rdh genes in response to specific chlorinated aromatic compounds. The regulators will be heterologously produced, biochemically characterized and the interaction with promoter regions of rdh genes and possible ligands studied. The genome sequence of the related but physiologically distinct Dehalococcoides strain DCMB5 will be annotated. Comparative analyzes of the genomes, rdh gene transcription and the proteomes of strains DCMB5 and CBDB1 will be carried out in close co-operation with other groups of the research cluster. The results will be correlated to the dechlorination behaviour of both strains in an attempt to assign functions to further key dehalogenases and their regulators and to identify possible regulatory cascades involved in the stepwise dechlorination of highly chlorinated compounds such as dibenzo-p-dioxins.

该项目的主要目的是表征还原脱卤酶(Rdh)基因表达的调控机制，使脱卤球菌能对多种氯有机化合物做出特异性反应，并进行多步脱氯反应。该项目将侧重于对脱氢球菌基因组中编码的可能调节蛋白的生化研究。菌株CBDB1通过基因差异转录为rdhA基因。将讨论Marr型调节器和双组分系统调节器的作用。根据多个rdh基因对特定氯代芳香族化合物的差异转录的观察，将选择假定的MAR型调控因子进行生化分析。这些调节剂将以异源方式生产，进行生化表征，并研究与rdh基因启动子区域和可能的配体之间的相互作用。相关的但生理上不同的脱卤球菌菌株DCMB5的基因组序列将被注释。将与研究小组的其他小组密切合作，对DCMB5和CBDB1菌株的基因组、rdh基因转录和蛋白质组进行比较分析。结果将与两个菌株的脱氯行为相关联，试图将功能分配给进一步的关键脱卤酶及其调节器，并确定在二苯并-对二恶英等高氯化合物的逐步脱氯过程中可能涉及的调节级联反应。

项目成果

Anaerobic microbial dehalogenation and its key players in the contaminated Bitterfeld‐Wolfen megasite

• DOI: 10.1093/femsec/fiy012
• 发表时间: 2018-04
• 期刊: FEMS Microbiology Ecology
• 影响因子: 4.2
• 作者: I. Nijenhuis;R. Stollberg;U. Lechner

The MarR-Type Regulator Rdh2R Regulates rdh Gene Transcription in Dehalococcoides mccartyi Strain CBDB1

• DOI: 10.1128/jb.00419-16
• 发表时间: 2016-12-01
• 期刊: JOURNAL OF BACTERIOLOGY
• 影响因子: 3.2
• 作者: Krasper, Lydia;Lilie, Hauke;Lechner, Ute
• 通讯作者: Lechner, Ute

Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi

• DOI: 10.1098/rstb.2012.0317
• 发表时间: 2013-04
• 期刊: Philosophical Transactions of the Royal Society B: Biological Sciences
• 作者: Anke Wagner;Lydia Segler;S. Kleinsteuber;G. Sawers;H. Smidt;U. Lechner

Dehalococcoides mccartyi Strain DCMB5 Respires a Broad Spectrum of Chlorinated Aromatic Compounds

• DOI: 10.1128/aem.02597-14
• 发表时间: 2014-11
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: Marlén Pöritz;Christian L. Schiffmann;G. Hause;Ulrike Heinemann;J. Seifert;N. Jehmlich;M. von Bergen;I. Nijenhuis;U. Lechner

Comparative Genomics and Transcriptomics of Organohalide-Respiring Bacteria and Regulation of rdh Gene Transcription

• DOI: 10.1007/978-3-662-49875-0_15
• 发表时间: 2016
• 作者: T. Kruse;H. Smidt;U. Lechner