Collaborative Research: Engineering yeast consortia for surface-display of complex cellulosome structures: A consolidated bioprocessing approach from cellulosic biomass to ethanol

合作研究：工程酵母菌群用于复杂纤维素体结构的表面展示：从纤维素生物质到乙醇的综合生物加工方法

• 批准号: 0903894
• 负责人: Nosang Myung
• 金额: $ 60万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0903894&HistoricalAwards=false
• 关键词: Collaborative; Research; Engineering; yeast; consortia

0903894ChenIntellectual MeritThe engineering strategy proposed emphasizes the efficiency of hydrolysis and synergy among cellulases, rather than focusing on the amount of enzymes produced or used. To emulate the success of a natural mechanism for efficient cellulose hydrolysis, complex cellulosomes (self-assembled multi-enzyme complexes) will be assembled on the yeast cell surface, enabling the ethanol-producing strain to utilize cellulose and concomitantly ferment it to ethanol. More importantly, by organizing these cellulases in an ordered structure, the enhanced synergy will increase the efficiency in hydrolysis, and thereby enhance ethanol production. The use of a single yeast strain for surface anchoring and cellulase secretion is unlikely to be successful again based on bioenergetic limitations. To solve this problem, a synthetic yeast consortium will be developed for the functional presentation of the complex cellulosome structures.Broader ImpactsEngineering microbes to utilize cellulose eliminates the need for cellulose treatment. If successful, this will allow vastly abundant low-cost agriculture residues to be used as raw materials for ethanol production. The enhanced value of crops leads to additional incomes to farmers, more efficient land use, and contributes to long-term agriculture sustainability. The increased production of ethanol reduces pollution to the environment and the need for imported petroleum. The integration of metabolic engineering strategies with the implementation of process technology represents a unique effort that expands the fundamental development of metabolic engineering into a practical remediation technology. The proposed research involves intersection of principles and methods of molecular genetics, synthetic biology, and fermentation. Graduate students participating in this research will gain an integrated perspective of the important interfaces and synergies connecting biochemistry, modern genetics, and process engineering. As the integration of research and education is one of the key programs of NSF, the proposed research will involve the participation of K-12 school students, undergraduates and graduate students, particularly from underrepresented groups.

0903894陈智贤提出的工程策略强调水解效率和纤维素酶之间的协同作用，而不是关注生产或使用的酶的数量。为了模仿有效纤维素水解的天然机制的成功，将在酵母细胞表面组装复杂的纤维素体（自组装的多酶复合物），使乙醇生产菌株能够利用纤维素并同时将其发酵成乙醇。更重要的是，通过将这些纤维素酶组织成有序结构，增强的协同作用将增加水解效率，从而提高乙醇产量。基于生物能量的限制，使用单一酵母菌株进行表面锚定和纤维素酶分泌不太可能再次成功。为了解决这一问题，将开发一种合成酵母菌群，用于复杂纤维素酶体结构的功能展示。更广泛的影响工程微生物利用纤维素消除了对纤维素处理的需要。如果成功，这将使大量低成本的农业残留物被用作乙醇生产的原材料。农作物价值的提高为农民带来了额外收入，提高了土地利用效率，并有助于农业的长期可持续性。乙醇产量的增加减少了对环境的污染和对进口石油的需求。代谢工程策略与过程技术的实施的整合代表了将代谢工程的基本发展扩展为实用修复技术的独特努力。该研究涉及分子遗传学、合成生物学和发酵的原理和方法的交叉。参与这项研究的研究生将获得连接生物化学，现代遗传学和工艺工程的重要接口和协同作用的综合视角。由于研究和教育的整合是NSF的关键项目之一，拟议的研究将涉及K-12学校学生，本科生和研究生的参与，特别是来自代表性不足的群体。